Transgenic mouse unit (C03)

• Subject Area: Immunology
• Term: from 2013 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 215346292

Genetically modified mice are crucial model systems for B-cell immunology. The Central Service Project C03 has provided transgenic / gene knockout technology for the whole TRR130, which was used for many projects. A new reporter mouse strain was developed, named B-VARIA mice, which allows tracking of B cell populations in vivo. In the next funding period, C03 will establish CRISPR/Cas9 for the generation of mice with defined mutations in several genes of the B cell lineage. The methodology for the application of CRISPR/Cas9 directly in zygotes will be established to induce multiple mutations in closely linked genes in a one-step reaction, to insert by homology dependent repair short loxP DNA sequences for conditional depletion of genes during certain phases of the B cell development and to use longer DNA homology fragments for the generation of complex knock-in alleles like reporter genes, immunoglobulin knock-in alleles, and other conditional alleles.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 130: B Cells: Immunity and Autoimmunity
• Applicant Institution: Friedrich-Alexander-Universität Erlangen-Nürnberg
• Project Heads: Professor Dr. Lars Nitschke; Professor Dr. Thomas Winkler