Raman spectroscopic monitoring of protein recognition by supramolecular ligands (A09)

• Subject Area: Theoretical Chemistry: Electronic Structure, Dynamics, Simulation ; Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry
• Term: from 2014 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 229838028

Project A9 aims at site-specific, sensitive and quantitative monitoring of protein recognition by supramolecular ligands using Raman spectroscopy. The SERS assay in SP1 realizes the idea of “controlled enhancement of multivalency by nanopositioning”: supramolecular ligands are positioned at defined distances and heights on the surface of a gold nanorod using a DNA origami platform in order to achieve significantly stronger binding to proteins. In SP2 we will establish UVRR spectroscopy as a generic tool for label-free, site-specific and quantitative monitoring of protein recognition by supramolecular ligands using a time-resolved approach.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1093: Supramolecular Chemistry on Proteins
• Applicant Institution: Universität Duisburg-Essen
• Project Head: Professor Dr. Sebastian Schlücker