Influenza-Sensitivität trotz scheinbar intakter Mx-Gene in Mus musculus castaneus

• Antragsteller: Professor Dr. Peter Stäheli
• Fachliche Zuordnung: Virologie
• Förderung: Förderung von 2014 bis 2018
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 249167911

• Erstellungsjahr: 2018

Zusammenfassung der Projektergebnisse

This project helped increasing our knowledge on the functional consequences of sequence variations in the MX1 protein of different Mus species. It showed that some MX1 variants acquired mutations that either reduce the metabolic stability of the mature protein or lower the binding affinity of MX1 for its viral target. This work forms a solid basis for future studies aimed at understanding why evolution select for MX1 variants with reduced activity in some murine species. At present this important question remains entirely unresolved.

Projektbezogene Publikationen (Auswahl)

• Functional comparison of Mx1 from two different mouse species reveals the involvement of loop L4 in the antiviral activity against influenza A viruses. J. Virol. 89: 10879-10890 (2015)
  Verhelst, J., Spitaels, J., Nürnberger, C., De Vlieger, D., Ysenbaert, T., Staeheli, P., Fiers, W., & X. Saelens
  
• (2016). Influenza virus susceptibility of wild-derived CAST/EiJ mice is determined by mutations in the Mx1 resistance gene. Doktorarbeit Fakultät für Biologie, Universität Freiburg
  Nürnberger, C.
  
• Influenza virus susceptibility of wildderived CAST/EiJ mice results from two amino acid changes in the MX1 restriction factor. J. Virol. 90: 10682-10692 (2016)
  Nürnberger, C., Zimmermann, V., Gerhardt, M., & P. Staeheli