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Projekt Druckansicht

Genetisch optimierte M13 Phagen als funktionalisierte biologische Template für die Herstellung bio/anorganischer nanostukturierter Materialien

Fachliche Zuordnung Herstellung und Eigenschaften von Funktionsmaterialien
Biomaterialien
Förderung Förderung von 2014 bis 2016
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 250530573
 
Erstellungsjahr 2019

Zusammenfassung der Projektergebnisse

The genetic modification of M13 phage is an essential step for the use, e.g. as a biotemplate for the fabrication of next generation optical and electronic devices. Such biotemplates open facile synthesis approaches for the fabrication of miniaturized devices. In this study, peptides that specifically bind to inorganic materials (gold and ZnO) were displayed on the phage by genetically engineering. There, it is possible to address specifically the localization on the phage surface: tip, body or tail. PCR technique enabled the generation of various mono- and bifunctional phages displaying either a gold and/or ZnO peptide on the surface of the phage. Phages expressing gold-binding peptides nucleate and grow gold nanoparticles on specific sites on the surface depending on the localization of the coat protein-peptide fusion. The phage templates expressing a ZnO-binding motif provided the base for the generation of multilayer assemblies. The inorganic ZnO phase was mineralized under soft reaction conditions. By the variation of reaction parameters a (002) textured polycrystalline thin film was prepared. Mulitlayer assemblies with 10 phage/ZnO layers can be prepared with a total thickness of >1 µm. Delicate TEM investigations showed the presence of the phage template in the multilayer assemblies and confirmed by TEM EDS analysis. The orientation of the phage templates in consecutive layers had only a minor effect on the mechanical properties of multilayer systems. However, the mechanical properties of such assemblies were controlled by the genetic constitution of the phage template. Based on the phage type either the Young’s modulus and hardness or the fracture toughness was efficiently increased. In addition to the mechanical properties, the piezoelectric behavior was controlled by the genetic constitution of the template. In this connection, the piezoelectric activity of single phage-ZnO hybridwires was induced by genetic engineering of the phage template, leading to the in situ poling of mineralized ZnO crystallites. Based on this phage-induced self-assembly process highly piezo-active nanowires were generated. The phage-templated biomineralization approach can be generalized for inorganic materials. The coating of irregularly shaped surfaces can be achieved using dip coating or spin coating process for preparing the organic template layer. Furthermore, single phage templates allow entering the field of nano- and microdevices.

Projektbezogene Publikationen (Auswahl)

 
 

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