Windows into the brain: Genetic analyses of TRP-channel function(s) in the homeostasis control center (P11)

• Subject Area: Molecular Biology and Physiology of Neurons and Glial Cells
• Term: since 2014
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 239283807

The median eminence (ME) is a circumventricular organ at the base of the brain and a central hub controlling body homeostasis. Using genetic labeling, we have identified populations of TRPA1 pars tuberalis cells, TRPC4 pericytes and TRPML3 tanycytes in the ME and will characterize the functional role of these cells in the mouse brain. Using transcriptional profiling and confocal Ca2+ imaging of acute brain slice preparations, we will I) characterize signaling pathways in these cells, II) analyze TRPA1, TRPC4 and TRPML3 channel function in this brain area via virally mediated CRISP/Cas conditional knock-out and III) pharmacogenetically activate these cells and analyze their contribution to body homeostasis.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 152: TRiPs to Homeostasis: Maintenance of Body Homeostasis by Transient Receptor Potential Channel Modules
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Ulrich Boehm