Assoziationsgenetische Kartierung von Resistenzen gegen die Netz- und Braunfleckenkrankheit in einem Gerstesortiment aus den Diversitätszentren
Zusammenfassung der Projektergebnisse
Net blotch and spot blotch are the most important diseases of barley and are present in all barley growing regions. Under optimal conditions, they cause high yield losses of 10% - 40% and reduce grain quality. The most cost-effective and environmentally friendly way to prevent losses is growing resistant cultivars. Molecular markers linked to effective resistance factors can accelerate the breeding process. Therfore, 449 barley accessions expressing different levels of resistance comprising landraces and commercial cultivars from the centres of diversity were selected. The set was phenotyped for seedling resistance to three isolates of each pathogen in controlled environments and for adult plant resistance at three field locations (Belarus/Russia, Germany and Australia), and genotyped with the 50k iSelect chip. Genome wide association studies (GWAS) were performed using 33,818 markers and a compressed mixed linear model (CMLM) to account for population structure and kinship. Overall, 254 significant marker-trait associations (MTA) corresponding to 15 distinct QTL regions were detected to be associated with resistance to the net form net blotch. For spot blotch, 38 significant MTAs were detected, which correspond to two loci. Furthermore, 25 KASP markers were designed based on the detected QTL and verified in six DH-populations. Four out of the 25 KASP markers were diagnostic in at least one of the DH- populations and can be used in marker assisted selection.
Projektbezogene Publikationen (Auswahl)
- (2016): Genome wide association studies for resistance to Pyrenophora teres f. teres and Cochliobolus sativus in barley (Hordeum vulgare). : The 12th International Barley Genetics Symposium, June 26-30, 2016, Minneapolis-St. Paul, Minnesota, United States of America, Poster: 138
Ordon, F., Novakazi, F.; Anisimova, A.; Afanasenko, O.; Kopahnke, D.
- (2016): Genomweite Assoziationsstudien zur Resistenz gegenüber Pyrenophora teres f. teres in Gerste (Hordeum vulgare). In: JKI (Hrsg.): 60. Deutsche Pflanzenschutztagung : 20. - 23. September 2016, Martin-Luther-Universität Halle-Wittenberg ; Kurzfassungen der Beiträge (Julius-Kühn-Archiv 454), Quedlinburg, 91-92
Novakazi, F.; Kopahnke, D.; Anisimova, A.; Afanasenko, O.; Ordon, F.
- (2017): Employing GWAS for identifying QTL for resistance to Pyrenophora teres f. teres and Cochliobolus sativus in barley (Hordeum vulgare). : 4th International Symposium on Genomics of Plant Genetic Resources (GPGR4), Giessen, Germany, Sept. 3-7, 2017, P42
Novakazi, F.; Anisimova, A.; Afanasenko, O.; Kopahnke, D.; Ordon, F.
- (2018): Association mapping for resistance to the net form of net blotch in a diverse barley set. In: Graner, A. (ed.): Book of Abstracts: GPBC 2018 - German Plant Breeding Conference, 28.02.-02.03.2018, Wernigerode, Leveraging the value of genomic information, 88
Novakazi, F.; Anisimova, A.; Afanasenko, O.; Kopahnke, D.; Ordon, F.
