Funktionale Bedeutung der Transmembranhelices und des Turrets für das Gating von Kaliumkanälen
Zusammenfassung der Projektergebnisse
The project has produced interesting results regarding: a) A so far unknown mechanism for a cytosolic gate in K+ channels that particularly stands out because of its unusual simplicity. b) A novel concept of regulation of gating by voltage. In the absence of a dedicated voltage-sensor domain, the extracellular turret is apparently able to confer voltagesensitivity. c) The possibility to determined site-specific ion occupation in the pore from electrophysiological data, which enabled the distinction of several candidate permeation models from MD simulations and the identification of a distinct sensor binding site in the selectivity filter. Additionally, the project generated incentive for new projects regarding the role of inner-helical hydrogen bonds for channel gating and the mechanisms of selectivity filter gating.
Projektbezogene Publikationen (Auswahl)
-
2015. How to resolve microsecond current fluctuations in single ion channels: The power of beta distributions. Channels. 9: 262–280
Schroeder, Indra
-
2016. A simple recipe for setting up the flux equations of cyclic and linear reaction schemes of ion transport with a high number of states: the arrow scheme. Channels. 10: 1–20
Hansen, Ulf-Peter; Rauh, Oliver & Schroeder, Indra
-
2017. Extended beta distributions open the access to fast gating in bilayer experiments-assigning the voltage-dependent gating to the selectivity filter. FEBS Lett. 591: 3850–3860
Rauh, Oliver; Hansen, Ulf‐Peter; Mach, Sebastian; Hartel, Andreas J.W.; Shepard, Kenneth L.; Thiel, Gerhard & Schroeder, Indra
-
2017. Identification of intra-helical bifurcated H-bonds as a new type of gate in K+ channels. J. Am. Chem. Soc. 139: 7494–7503
Rauh, Oliver; Urban, Martin; Henkes, Leonhard M.; Winterstein, Tobias; Greiner, Timo; Van Etten, James L.; Moroni, Anna; Kast, Stefan M.; Thiel, Gerhard & Schroeder, Indra
-
2018. Molecular explanations for gating in simple model K+ channels. PhD thesis, Tech. Univ. Darmstadt
Rauh, O.
-
2018. Reconstitution and functional characterization of ion channels from nanodiscs in lipid bilayers. J. Gen. Physiol. 150: 637–646
Winterstein, Laura-Marie; Kukovetz, Kerri; Rauh, Oliver; Turman, Daniel L.; Braun, Christian; Moroni, Anna; Schroeder, Indra & Thiel, Gerhard
-
2018. Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes. Proc. Natl. Acad. Sci. 115: E1789–E1798
Hartel, Andreas J. W.; Ong, Peijie; Schroeder, Indra; Giese, M. Hunter; Shekar, Siddharth; Clarke, Oliver B.; Zalk, Ran; Marks, Andrew R.; Hendrickson, Wayne A. & Shepard, Kenneth L.
-
2018. Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral K+ channel. Sci. Rep. 8: 10406
Rauh, O.; Hansen, U. P.; Scheub, D. D.; Thiel, G. & Schroeder, I.
-
2019. High bandwidth approaches in nanopore and ion channel recordings - A tutorial review. Anal. Chim. Acta. 1061: 13–27
Hartel, Andreas J.W.; Shekar, Siddharth; Ong, Peijie; Schroeder, Indra; Thiel, Gerhard & Shepard, Kenneth L.
