Polymere Nanocarrier zur Visualisierung und Quantifizierung von molekularen Freisetzungen
Zusammenfassung der Projektergebnisse
The aim of our project was the establishment of biocompatible release platforms, capable of providing a handle for real-time readout of molecular release efficiency. In summary, we have established: (i) A water-soluble and biocompatible carrier based on polymethacrylates allowing the immobilization and triggered release of Dox. (ii) The successful demonstration of fluorescence-based methods for real-time quantification of molecular release. (iii) A significant level of bioapplicability of the novel release- and reporter platform, established in three-dimensional cell culture studies. (iv) The establishment of visible-light chemistry on a spin-silenced nanocarrier as a platform for the quantification of temporally and spatially resolved release. (v) The investigative relevance of novel microfluidic 3D-cell culture systems as a replacement for traditional animal model studies, as well as a method to automatically evaluate results obtained from such systems.
Projektbezogene Publikationen (Auswahl)
- A Polymeric Nanocarrier for the Visualization and Quantification of Molecular Release, 8th Pacific Symposium on Radical Chemistry (PSRC-8), 18th to 22nd of July 2017, Brisbane, Australia
C. Barner-Kowollik, J. P. Blinco, M. Eing, K. Fairfull-Smith
- Polym. Chem. 2017, 8, 6199-6203
H. Mutlu, C. W. Schmitt, N. Wedler-Jasinski, H. Woehlk, K. E. Fairfull-Smith, J. P. Blinco, C. Barner-Kowollik
(Siehe online unter https://doi.org/10.1039/c7py01437f) - Quantification of Molecular Drug Release via Unpaired Spin Fluorescence Silencing. 8th Pacific Symposium on Radical Chemistry (PSRC-8), 18th to 22nd of July 2017, Brisbane, Australia
C. Barner-Kowollik, J. P. Blinco, M. Eing, K. Fairfull-Smith
- Chem. Eur. J., 2018, 24, 12246-12249
M. Eing, B. Tuten, J. P. Blinco and C. Barner-Kowollik
(Siehe online unter https://doi.org/10.1002/chem.201800732) - Polym. Chem., 2018, 9, 499–505
M. Eing, B. Olshausen, K. E. Fairfull-Smith, U. Schepers, C. Barner-Kowollik and J. P. Blinco
(Siehe online unter https://doi.org/10.1039/c7py01942d) - Spin Fluorescence Silencing: A Powerful Tool for Imaging Molecular Release. Bordeaux Polymer Conference, May 28-31th, 2018, Bordeaux, France
H. Mutlu, C. W. Schmitt, M. Eing, K. Fairfull-Smith, J. P. Blinco, C. Barner-Kowollik
- “Shining bright: Fluorescent tools for drug discovery, development and delivery”, KIT, 2018, Cuvillier Verlag, ISBN: 9783736998162
Bettina Olshausen
- “Polymeric Nanocarriers for the Visualisation and Quantification of Molecular Release”, Dissertation – KIT
M. Eing
