Polymeric Nanocarriers for the Visualization and Quantification of Molecular Release

• Applicants: Professor Dr. Christopher Barner-Kowollik; Professorin Dr. Ute Schepers
• Subject Area: Preparatory and Physical Chemistry of Polymers
• Term: from 2014 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 265519003

• Final Report Year: 2019

Final Report Abstract

The aim of our project was the establishment of biocompatible release platforms, capable of providing a handle for real-time readout of molecular release efficiency. In summary, we have established: (i) A water-soluble and biocompatible carrier based on polymethacrylates allowing the immobilization and triggered release of Dox. (ii) The successful demonstration of fluorescence-based methods for real-time quantification of molecular release. (iii) A significant level of bioapplicability of the novel release- and reporter platform, established in three-dimensional cell culture studies. (iv) The establishment of visible-light chemistry on a spin-silenced nanocarrier as a platform for the quantification of temporally and spatially resolved release. (v) The investigative relevance of novel microfluidic 3D-cell culture systems as a replacement for traditional animal model studies, as well as a method to automatically evaluate results obtained from such systems.

Publications

• A Polymeric Nanocarrier for the Visualization and Quantification of Molecular Release, 8th Pacific Symposium on Radical Chemistry (PSRC-8), 18th to 22nd of July 2017, Brisbane, Australia
  C. Barner-Kowollik, J. P. Blinco, M. Eing, K. Fairfull-Smith
  
• Polym. Chem. 2017, 8, 6199-6203
  H. Mutlu, C. W. Schmitt, N. Wedler-Jasinski, H. Woehlk, K. E. Fairfull-Smith, J. P. Blinco, C. Barner-Kowollik
  
• Quantification of Molecular Drug Release via Unpaired Spin Fluorescence Silencing. 8th Pacific Symposium on Radical Chemistry (PSRC-8), 18th to 22nd of July 2017, Brisbane, Australia
  C. Barner-Kowollik, J. P. Blinco, M. Eing, K. Fairfull-Smith
  
• Chem. Eur. J., 2018, 24, 12246-12249
  M. Eing, B. Tuten, J. P. Blinco and C. Barner-Kowollik
  
• Polym. Chem., 2018, 9, 499–505
  M. Eing, B. Olshausen, K. E. Fairfull-Smith, U. Schepers, C. Barner-Kowollik and J. P. Blinco
  
• Spin Fluorescence Silencing: A Powerful Tool for Imaging Molecular Release. Bordeaux Polymer Conference, May 28-31th, 2018, Bordeaux, France
  H. Mutlu, C. W. Schmitt, M. Eing, K. Fairfull-Smith, J. P. Blinco, C. Barner-Kowollik
  
• “Shining bright: Fluorescent tools for drug discovery, development and delivery”, KIT, 2018, Cuvillier Verlag, ISBN: 9783736998162
  Bettina Olshausen
  
• “Polymeric Nanocarriers for the Visualisation and Quantification of Molecular Release”, Dissertation – KIT
  M. Eing