Die Bedeutung von Selenonein im humanen Selenmetabolismus
Zusammenfassung der Projektergebnisse
Overall, the complementary expertise of the analytical chemists in Graz and the toxicologists in Potsdam led to the isolation of selenoneine and the elucidation of its possible transport mechanisms, metabolic changes and modes of action in comparison to other naturally occurring small selenium species. The in vitro transfer studies applied in this project provide evidence that selenoneine efficiently transfers across the intestinal barrier. In contrast, selenoneine is likely to have a slow transfer rate to the brain and seems not to be metabolised by the brain endothelial cells. Our studies in cultured human cells and in C.elegans indicate that selenoneine is unlikely to be involved into the Se metabolic cycle. At the same time, sulfur to Se exchange in the erhothioneine structure led to a substantial increase in the radical scavenging activity, which raises the question of the potential role of selenoneine as a highly effective dietary antioxidant.
Projektbezogene Publikationen (Auswahl)
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(2016) Differing cytotoxicity and bioavailability of selenite, methylselenocysteine, selenomethionine, selenosugar 1 and trimethylselenonium ion and their underlying metabolic transformations in human cells. Mol. Nutr. Food Res. 60, 2622-2632
Marschall T.A., Bornhorst J., Kuehnelt D., Schwerdtle T.
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(2017) Quantitative determination of the sulfur-containing antioxidant ergothioneine by HPLC/ICPQQQ- MS. J. Anal. At. Spectrom. 32, 1571-1581
Kroepfl N., Marschall T.A., Francesconi, Francesconi K.A., Schwerdtle T., Kuehnelt D.
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(2017) Tracing cytotoxic effects of small organic Se species in human liver cells back to total cellular Se and Se metabolites. Metallomics 9, 268-277
Marschall T.A., Kroepfl N., Jensen K.B., Bornhorst J., Meermann B., Kuehnelt D., Schwerdtle T.
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(2018) Biosynthesis and isolation of selenoneine from genetically modified fission yeast Metallomics 10, 1532-1538
Turrini N.G., Kroepfl N., Jensen K.B., Reiter T.C., Francesconi K.A., Schwerdtle T., Kroutil W., Kuehnelt D.
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(2018) Selenium species-dependent toxicity, bioavailability and metabolic transformations in Caenorhabditis elegans. Metallomics 10, 818-827
Rohn I., Marschall T.A., Kroepfl N., Jensen K.B., Aschner M., Tuck S., Kuehnelt D., Schwerdtle T., Bornhorst J.
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(2019) Selenoneine ameliorates peroxide-induced oxidative stress in C. elegans. J. Trace Elem. Med. Biol. 55, 78-81
Rohn I., Kroepfl N., Aschner M., Bornhorst J., Kuehnelt D., Schwerdtle T.
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(2019) Side-directed transfer and presystemic metabolism of selenoneine in a human intestinal barrier model. Mol. Nutr. Food Res. 63, Article Number: 1900080
Rohn I., Kroepfl N., Bornhorst J., Kuehnelt D., Schwerdtle T.
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(2019) Treatment of Caenorhabditis elegans with small selenium species enhances antioxidant defense systems. Mol. Nutr. Food Res. 63
Rohn I., Raschke S., Aschner M., Tuck S., Kuehnelt D., Kipp A., Schwerdtle T., Bornhorst J.
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(2021) Capabilities of selenoneine to cross the in vitro blood-brain barrier model. Metallomics, 13 (1)
Drobyshev E., Raschke S., Glabonjat R.A., Bornhorst J., Ebert F., Kuehnelt D., Schwerdtle T.
