Regulatorische kleine RNAs aus den 3' Regionen von mRNAs

• Antragsteller: Professor Dr. Jörg Vogel
• Fachliche Zuordnung: Stoffwechselphysiologie, Biochemie und Genetik der Mikroorganismen
• Förderung: Förderung von 2015 bis 2019
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 278016524

• Erstellungsjahr: 2020

Zusammenfassung der Projektergebnisse

Our discoveries with the CpxQ and SdhX sRNAs have very robustly shown that the 3′ regions of mRNA genes constitute a large reservoir from which the Hfq network recruits new sRNAs to rewire gene expression at the post-transcriptional level. Through our studies of these two 3’UTR-derived sRNAs, we have firmly established the general principle of their biogenesis by RNase E and that they fulfill as important and conserved functions in bacterial cells as do sRNAs transcribed from otherwise “empty” intergenic regions. The short SdhX sRNA variant identified by us lends itself to being exploited both, as a molecular platform for functional seed regions and as a prime candidate for the outstanding high-resolution structure of Hfq with a sRNA.

Projektbezogene Publikationen (Auswahl)

• (2020) Trans-Acting Small RNAs and Their Effects on Gene Expression in Escherichia coli and Salmonella enterica. EcoSal Plus 9 (1) 1-24
  Hör, Jens; Matera, Gianluca; Vogel, Jörg; Gottesman, Susan; Storz, Gisela
  (Siehe online unter https://doi.org/10.1128/ecosalplus.ESP-0030-2019)
• (2016) A 3’UTR derived small RNA provides the regulatory noncoding arm of the inner membrane stress response. Molecular Cell 61:352-363
  Chao Y, Vogel J
  (Siehe online unter https://doi.org/10.1016/j.molcel.2015.12.023)
• (2017) Molecular mechanism of mRNA repression in trans by a ProQ-dependent small RNA. EMBO Journal 36(8):1029-1045
  Smirnov A, Wang C, Drewry LL, Vogel J
  (Siehe online unter https://doi.org/10.15252/embj.201696127)
• (2019) Functional expansion of a TCA cycle operon mRNA by a 3’ end-derived small RNA. Nucleic Acids Research 47(4):2075-2088
  Miyakoshi M, Matera G, Maki K, Sone Y, Vogel J
  (Siehe online unter https://doi.org/10.1093/nar/gky1243)
• (2019) The major RNA-binding protein ProQ impacts virulence gene expression in Salmonella Typhimurium. mBio 10(1) pii:e02504-18
  Westermann AJ, Venturini E, Sellin ME, Förstner KU, Hardt WD, Vogel J
  (Siehe online unter https://doi.org/10.1128/mBio.02504-18)
• (2020) The conserved 3’ UTR-derived small RNA NarS mediates mRNA crossregulation during nitrate respiration. Nucleic Acids Research 48(4):2126–2143
  Wang C, Chao Y, Matera G, Gao Q, Vogel J
  (Siehe online unter https://doi.org/10.1093/nar/gkz1168)