Structure and function studies of the human voltage-gated proton channel HV1

• Applicant: Professor Dr. Boris Musset
• Subject Area: Biochemistry ; Anatomy and Physiology ; Biophysics
• Term: from 2015 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 279044618

The voltage-gated proton channel Hv1 has many implications in physiology. The by far best understood is the charge compensation during the oxidative burst in phagocytosis. Recent publications found additional physiological importance of Hv1 in metastasized breast, colon and rectal cancer cells, sperm, B-cells, microglia, and Basophiles. These implications turn Hv1 into a promising target, to design drugs against cancer, infertility, stroke and immune malfunction.We will investigate the structure and function of Hv1. Therefore we will use the patch clamp technique combined with molecular dynamics simulations to generate and refine a homology model of hHv1. The computer model will be the basis to enhance rational drug design of Hv1. Furthermore we are able to test the efficiency of new drugs directly on expression systems and native cells.

• DFG Programme: Research Grants