Substrate binding and cleavage mechanism of rubber oxygenase RoxA

• Applicant: Professor Dr. Dieter Jendrossek
• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2015 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 281987579

• Final Report Year: 2019

Final Report Abstract

Das Projekt verlief äußerst erfolgreich: zusammen mit dem ebenfalls von der DFG geförderten Parallelprojekt konnten Details zu den Mechanismen der enzymatischen Polyisoprenspaltung anhand von drei model-rubber oxygenases erarbeitet werden.

Publications

• (2017) Assays for the Detection of Rubber Oxygenase Activities. Bioprotocol 7:1–14
  Röther W, Birke J, Jendrossek D
  (See online at https://doi.org/10.21769/BioProtoc.2188)
• (2017) Production of functionalized oligo-isoprenoids by enzymatic cleavage of rubber. Microb Biotechnol 43:1238
  Röther W, Birke J, Grond S, Beltran JM, Jendrossek D
  (See online at https://doi.org/10.1111/1751-7915.12748)
• (2017) RoxB Is a Novel Type of Rubber Oxygenase That Combines Properties of Rubber Oxygenase RoxA and Latex Clearing Protein (Lcp). Appl Environ Microbiol 83:e00721–17
  Birke J, Röther W, Jendrossek D
  (See online at https://doi.org/10.1128/AEM.00721-17)
• (2018) Metabolic and taxonomic insights into the Gram-negative natural rubber degrading bacterium Steroidobacter cummioxidans sp. nov., strain 35Y. PLoS ONE 13:e0197448
  Sharma V, Siedenburg G, Birke J, Mobeen F, Jendrossek D, Prakash T
  (See online at https://doi.org/10.1371/journal.pone.0197448)
• (2018) Rhizobacter gummiphilus NS21 has two rubber oxygenases (RoxA and RoxB) acting synergistically in rubber utilisation. Appl Microbiol Biotechnol 241:184–13
  Birke J, Röther W, Jendrossek D
  (See online at https://doi.org/10.1007/s00253-018-9341-6)
• (2018) Rubber oxygenases. Appl Microbiol Biotechnol 78:4543–18
  Jendrossek D, Birke J
  (See online at https://doi.org/10.1007/s00253-018-9453-z)