How transcription factors regulate bacterial RNA polymerase activity

• Applicant: Professor Dr. Paul Rösch
• Subject Area: Structural Biology
• Term: from 2015 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 284071855

• Final Report Year: 2023

No abstract available

Publications

• Determination of RNA polymerase binding surfaces of transcription factors by NMR spectroscopy. Scientific Reports, 5(1).
  Drögemüller, Johanna; Strauß, Martin; Schweimer, Kristian; Jurk, Marcel; Rösch, Paul & Knauer, Stefan H.
  
• The two domains of Mycobacterium tuberculosis NusG protein are dynamically independent. Journal of Biomolecular Structure and Dynamics, 34(2), 352-361.
  Strauß, Martin; Schweimer, Kristian; Burmann, Björn M.; Richter, Anne; Güttler, Stephanie; Wöhrl, Birgitta M. & Rösch, Paul
  
• Thermotoga maritima NusG: domain interaction mediates autoinhibition and thermostability. Nucleic Acids Research, 45(1), 446-460.
  Drögemüller, Johanna; Schneider, Christin; Schweimer, Kristian; Strauß, Martin; Wöhrl, Birgitta M.; Rösch, Paul & Knauer, Stefan H.
  
• Transcription is regulated by NusA:NusG interaction. Nucleic Acids Research, 44(12), 5971-5982.
  Strauß, Martin; Vitiello, Christal; Schweimer, Kristian; Gottesman, Max; Rösch, Paul & Knauer, Stefan H.
  
• Structure and nucleic acid binding properties of KOW domains 4 and 6–7 of human transcription elongation factor DSIF. Scientific Reports, 8(1).
  Zuber, Philipp K.; Hahn, Lukas; Reinl, Anne; Schweimer, Kristian; Knauer, Stefan H.; Gottesman, Max E.; Rösch, Paul & Wöhrl, Birgitta M.
  
• The universally-conserved transcription factor RfaH is recruited to a hairpin structure of the non-template DNA strand. eLife, 7.
  Zuber, Philipp K.; Artsimovitch, Irina; NandyMazumdar, Monali; Liu, Zhaokun; Nedialkov, Yuri; Schweimer, Kristian; Rösch, Paul & Knauer, Stefan H.
  
• Ancient Transcription Factors in the News. mBio, 10(1).
  Artsimovitch, Irina & Knauer, Stefan H.
  
• Reversible fold-switching controls the functional cycle of the antitermination factor RfaH. Nature Communications, 10(1).
  Zuber, Philipp Konrad; Schweimer, Kristian; Rösch, Paul; Artsimovitch, Irina & Knauer, Stefan H.
  
• SuhB is an integral part of the ribosomal antitermination complex and interacts with NusA. Nucleic Acids Research, 47(12), 6504-6518.
  Dudenhoeffer, Benjamin R.; Schneider, Hans; Schweimer, Kristian & Knauer, Stefan H.
  
• Differential Local Stability Governs the Metamorphic Fold Switch of Bacterial Virulence Factor RfaH. Biophysical Journal, 118(1), 96-104.
  Galaz-Davison, Pablo; Molina, José Alejandro; Silletti, Steve; Komives, Elizabeth A.; Knauer, Stefan H.; Artsimovitch, Irina & Ramírez-Sarmiento, César A.
  
• Escherichia coli NusG Links the Lead Ribosome with the Transcription Elongation Complex. iScience, 23(8), 101352.
  Washburn, Robert S.; Zuber, Philipp K.; Sun, Ming; Hashem, Yaser; Shen, Bingxin; Li, Wen; Harvey, Sho; Acosta, Reyes Francisco J.; Gottesman, Max E.; Knauer, Stefan H. & Frank, Joachim
  
• Structural and thermodynamic analyses of the β-to-α transformation in RfaH reveal principles of fold-switching proteins. eLife, 11.
  Zuber, Philipp K.; Daviter, Tina; Heißmann, Ramona; Persau, Ulrike; Schweimer, Kristian & Knauer, Stefan H.