The function of CLEC-1 in the skin
Parasitology and Biology of Tropical Infectious Disease Pathogens
Final Report Abstract
In this research project I was able to fulfill all my short term goals set in the proposal: (1) I increased my scientific visibility documented by the invitation to review a scientific publication, (2) I gained knowledge in state-of-the-art technologies and increased my skill set (3) I increased my scientific network (e.g. collaboration with Dr Martin Stacey, University of Leeds on Il-36) and (4) gained intense expertise in fungal immunology. I set the basis for my own independent research in the field of dermatophytes, a research topic hardly addressed in the scientific community. In the first 6 month I had to adapt and change the focus of the whole project because of the inability to detect MelLec (Clec-1) in the quiescent skin. I drafted a new project with the title: Assessment of the cutaneous immune response during Trichophyton rubrum infection using an experimental mouse model. A significant progress was made in this project: I established an in vivo skin infection model with the anthropophilic dermatophyte T. rubrum, I established a single cell isolation protocol for flow cytometry of mouse skin, investigated the binding pattern of different C-type lectin receptors to T. rubrum conidia and generated an antibody against human MelLec. Thus, the basis for an independent research project was made.
Publications
- Antifungal defense of probiotic Lactobacillus rhamnosus GG is mediated by blocking adhesion and nutrient depletion. PLoS One. 2017 Oct 12;12(10):e0184438
Mailänder-Sánchez D, Braunsdorf C, Grumaz C, Müller C, Lorenz S, Stevens P, Wagener J, Hebecker B, Hube B, Bracher F, Sohn K, Schaller M
(See online at https://doi.org/10.1371/journal.pone.0184438) - Candida albicans-Induced Epithelial Damage Mediates Translocation through Intestinal Barriers. MBio. 2018 Jun 5;9(3)
Allert S, Förster TM, Svensson CM, Richardson JP, Pawlik T, Hebecker B, Rudolphi S, Juraschitz M, Schaller M, Blagojevic M, Morschhäuser J, Figge MT, Jacobsen ID, Naglik JR, Kasper L, Mogavero S, Hube B
(See online at https://doi.org/10.1128/mBio.00915-18) - Recognition of DHN-melanin by a C-type lectin receptor is required for immunity to Aspergillus. Nature. 2018 Mar 15;555(7696):382-386
Stappers MHT, Clark AE, Aimanianda V, Bidula S, Reid DM, Asamaphan P, Hardison SE, Dambuza IM, Valsecchi I, Kerscher B, Plato A, Wallace CA, Yuecel R, Hebecker B, da Glória Teixeira Sousa M, Cunha C, Liu Y, Feizi T, Brakhage AA, Kwon-Chung KJ, Gow NAR, Zanda M, Piras M, Zanato C, Jaeger M, Netea MG, van de Veerdonk FL, Lacerda JF, Campos A, Carvalho A, Willment JA, Latgé JP, Brown GD
(See online at https://doi.org/10.1038/nature25974)
