Analysis of the role of FASCIATED EAR2 (FEA2) in a signaling pathway that restricts meristem growth in maize
Final Report Abstract
The proposed biochemical approach to characterize the FEA2 receptor complex, using a transgenic FEA2 tandem affinity purification tag line, didn't provide any results. Based on the fact that both GFP-tagged lines didn't provide results either, and that any of our FEA2-tagged transgenic maize lines had the ability to complement the fea2-0 mutation, I decided to stop pursuing these biochemical approaches. However, due to intensive trouble-shooting with the head of the Cold Spring Harbor Laboratory Proteomics facility, Dr. Derryl Pappin, I am confident, that I improved my knowledge about protein purification and immuno-precipitation approaches, including the subsequent analysis of mass-spec derived data. This will be very useful for the intended isolation of the CT2-containing protein complex. The fact that the CT2-YFP fusion has already been proven to complement the cf2-mutant makes it a suitable resource for identifying novel member of Ga-signaling in maize, which is so far poorly understood. In addition it will be very interesting to analyze the identified genetic interaction between FEA2- and CT2-signallng with respect to meristem size regulation.
Publications
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„Analysis of signaling pathways regulating meristem size in maize". 24th Missouri Plant Biol. Symp., Columbia, MO, USA
Bommert P., Wang J., Newman L., Guo M., Bruce W. and D. Jackson
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„Analysis of meristem size in maize". 50th Maize Genetics Conference, Washington D.C, USA
Bommert P. and D. Jackson
