Analysis of the role of FASCIATED EAR2 (FEA2) in a signaling pathway that restricts meristem growth in maize
Final Report Abstract
The proposed biochemical approach to characterize the FEA2 receptor complex, using a transgenic FEA2 tandem affinity purification tag line, didn't provide any results. Based on the fact that both GFP-tagged lines didn't provide results either, and that any of our FEA2-tagged transgenic maize lines had the ability to complement the fea2-0 mutation, I decided to stop pursuing these biochemical approaches. However, due to intensive trouble-shooting with the head of the Cold Spring Harbor Laboratory Proteomics facility, Dr. Derryl Pappin, I am confident, that I improved my knowledge about protein purification and immuno-precipitation approaches, including the subsequent analysis of mass-spec derived data. This will be very useful for the intended isolation of the CT2-containing protein complex. The fact that the CT2-YFP fusion has already been proven to complement the cf2-mutant makes it a suitable resource for identifying novel member of Ga-signaling in maize, which is so far poorly understood. In addition it will be very interesting to analyze the identified genetic interaction between FEA2- and CT2-signallng with respect to meristem size regulation.
Publications
- 2007 „Analysis of signaling pathways regulating meristem size in maize". 24th Missouri Plant Biol. Symp., Columbia, MO, USA
Bommert P., Wang J., Newman L., Guo M., Bruce W. and D. Jackson
- 2008 „Analysis of meristem size in maize". 50th Maize Genetics Conference, Washington D.C, USA
Bommert P. and D. Jackson
