Verhalten von Proteinen in mikrofluidischen Kanälen
Zusammenfassung der Projektergebnisse
We have proposed the sensor approach that combines acoustic and electrical methods for label-free separation and detection of proteins for potential application in routine urinalysis diagnostics. The method is based on near the surface detection with shear bulk acoustic resonator approach with structured surface for enhanced mass density sensing. The applied electrical field allows to modulate the surface concentration of targeting protein and detect its concentration. The results of numerical studies show the ability of proposed sensor concept to separate and specifically detect oligomeric forms of uromodulin, albumin and globulin. lnteraction in acoustic and electric fields allows to amplify the sensor response to the presence of targeting protein but the values of expected frequency shift vividly show the requirement of high Q factor resonant biosensor performance. lt was demonstrated that sensing technology based on fast-protein Iiquid chromatography has great potential for developing low-cost, multiple-use, optical sensors for determining protein concentrations in biological fluids in the framework of point-of-care testing systems. lt was shown in the clinical trial involving effluent peritoneal dialysate samples that the errors in the measurements are comparable to routine methods and are acceptable for POCT devices. Since the FPLC instruments rely on reusable, affordable chromatographic columns and utilize only minimal amounts of very cheap chemicals, they may significantly reduce medical costs, especially for patients with severe chronic conditions requiring frequent testing. After overcoming several technical and methodological problems, FPLC sensors could be implemented in clinical practice for both home and hospital use.
Projektbezogene Publikationen (Auswahl)
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Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications. Nanotechnology, Science and Applications, Volume 11(c(2018, 4)), 15-21.
Kurochkina, Margarita; Konshina, Elena; Oseev, Aleksandr & Hirsch, Soeren
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Label-Free Protein Detection by Micro-Acoustic Biosensor Coupled with Electrical Field Sorting. Theoretical Study in Urine Models. Sensors, 21(7), 2555.
Mukhin, Nikolay; Konoplev, Georgii; Oseev, Aleksandr; Schmidt, Marc-Peter; Stepanova, Oksana; Kozyrev, Andrey; Dmitriev, Alexander & Hirsch, Soeren
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Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures. Biomedicines, 10(2), 207.
Konoplev, Georgii; Agafonova, Darina; Bakhchova, Liubov; Mukhin, Nikolay; Kurachkina, Marharyta; Schmidt, Marc-Peter; Verlov, Nikolay; Sidorov, Alexander; Oseev, Aleksandr; Stepanova, Oksana; Kozyrev, Andrey; Dmitriev, Alexander & Hirsch, Soeren
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Optical Chemical Sensor Based on Fast-Protein Liquid Chromatography for Regular Peritoneal Protein Loss Assessment in End-Stage Renal Disease Patients on Continuous Ambulatory Peritoneal Dialysis. Chemosensors, 10(6), 232.
Kuznetsov, Artur; Frorip, Aleksandr; Sünter, Alar; Korsakov, Vadim; Konoplev, Georgii; Stepanova, Oksana; Roschina, Natalia; Ovsyannikov, Nikolay; Lialin, Daniil; Gerasimchuk, Roman; Dmitriev, Alexander; Mukhin, Nikolay & Hirsch, Soeren
