Molecular mechanisms of ribosome associated enzymes in stress response (22)

• Subject Area: Structural Biology
• Term: from 2016 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 201348542

N-terminal acetylation (NTA) is the most abundant protein modification of cytosolic proteins in eukaryotes with the acetylome fluctuating upon cellular stress. We continue to dissect N-acetyltransferases (NATs; NatA, NatC) and their role in protein quality control using an integrative structural biology approach combined with in vitro and in vivo assays. We will explore the molecular mechanisms of NTA in model organisms and whether the bi-functional activity of NATs as KATs (N-acetyltransferase) adds another layer of regulation. Analysis of NATs and other cotranslational factors will finally allow us to unravel their interplay at the ribosomal tunnel exit.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1036: Cellular Surveillance and Damage Response
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Head: Professorin Dr. Irmgard Sinning