Co-translational membrane targeting by SRP and FlhF (P13)

• Subject Area: Cell Biology
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 269423233

The assembly of the C-ring, a crucial cytoplasmic part of the bacterial flagellum, can be reconsti-tuted in vitro in the presence of FlhF and its regulator ATPase FlhG for the S. putrefaciens system. We will continue to elaborate this in vitro system to dissect the C-ring assembly process and characterize the involvement of FlhF and FlhG by cryo-electron microscopy (cryo-EM). In addition, we will purify and characterize mainly by cryo-EM one of the crucial polar landmark proteins, HubP, which establishes correct spatiotemporal patterns in the monotrichous S. putrefaciens, for example by recruiting FlhF to the pole region.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 174: Spatiotemporal dynamics of bacterial cells
• Applicant Institution: Philipps-Universität Marburg
• Project Head: Professor Dr. Roland Beckmann