Dynamic focussing photoacoustic microscopy of angiogenesis in vivo
Final Report Abstract
Optical resolution photoacoustic microscopy (OR-PAM) is an imaging technique that combines the high spatial resolution of optical microscopy with specificity for endogenous contrast agents such as hemoglobin. Because it allows functional, quantitative imaging of, for example, blood flow and blood oxygenation, it has great potential as a valuable research tool for vascular biology. In this work, an OR-PAM system with a planar Fabry-Pérot (FP) ultrasound sensor was developed for functional imaging of the vasculature in small animal models. The technological innovations involve both the FP sensors developed and the optics of the OR-PAM system. A novel planar FP sensor was developed as part of the project. Different polymers and film thicknesses were tested for the development. The transparency of the FP sensor to wavelengths in the visible and near-infrared spectrum enables the integration of the developed OR-PAM system with other optical imaging modalities such as multiphoton microscopy, which could enable simultaneous morphological imaging of endothelial cells inside blood vessels and functional imaging of blood. The developed OR-PAM system features a reverse mode, broadband ultrasound detection, and high acoustic sensitivity by minimizing the distance between the source of the photoacoustic (PA) signals and the FP detector. The 3D imaging capabilities of the system were demonstrated on a leaf skeleton phantom and a live zebrafish embryo. For spectroscopic imaging of blood oxygenation, the system incorporates multi-wavelength excitation, which was tested in ink phantoms. For quantitative imaging of blood flow velocity, a fast imaging mode was developed that achieves a scan rate of 100,000 A-lines/s. In summary, the developed system paves the way to make OR-PAM a useful tool for vascular biology applications and ultimately for medical research.
Publications
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Backward-mode Optical-Resolution Photoacoustic Microscopy Using a Planar Fabry-Pérot Ultrasound Sensor. SPIE Photonics West 2019, San Francisco, USA. Posterpräsentation
Ulrike Pohle, Elisabeth Baumann, Silvio Pulwer, Claus Villringer, Edward Zhang, Holger Gerhardt & Jan Laufer
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Backward-mode optical-resolution photoacoustic microscope for funtional imaging using a planar Fabry-Pérot ultrasound sensor. SPIE Photonics West 2020, San Francisco, USA. Posterpräsentation
Elisabeth Baumann, Ulrike Pohle, Thomas Allen, Edward Zhang, Holger Gerhardt & Jan Laufer
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Fast optical and acoustic-resolution photoacoustic microscopy using structured interrogation of a Fabry-Pérot sensor. SPIE Photonics West 2020, San Francisco, USA. Konferenzvortrag
Ulrike Pohle, Elisabeth Baumann, Claus Villringer, Silvio Pulwer, Edward Zhang, Thomas J. Allen, Robert Nuster & Jan Laufer
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A backward-mode optical-resolution photoacoustic microscope for 3D imaging using a planar Fabry-Pérot sensor. Photoacoustics, 24, 100293.
Baumann, Elisabeth; Pohle, Ulrike; Zhang, Edward; Allen, Thomas; Villringer, Claus; Pulwer, Silvio; Gerhardt, Holger & Laufer, Jan
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A backward-mode optical-resolution photoacoustic microscope for functional 3D imaging. DPG Frühjahrstagung SKM 2023, Dresden, Germany. Posterpräsentation
Elisabeth Baumann, Ulrike Pohle, Thomas Allen, Holger Gerhardt & Jan Laufer
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Evaluation of fabrication methods for Fabry-Perot polymer film ultrasound sensors. Opto-Acoustic Methods and Applications in Biophotonics VI, 33. SPIE.
Lebek, Werner; Heyroth, Frank; Syrowatka, Frank; Villringer, Claus; Goerlitz, Sylvia & Laufer, Jan
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Reconstructing stochastic cell population trajectories reveals regulators and heterogeneity of endothelial flow-migration coupling driving vascular remodelling. Cold Spring Harbor Laboratory.
Giese, Wolfgang; Rosa, André; Baumann, Elisabeth; Oppenheim, Olya; Akmeric, Emir B.; Andrade, Santiago; Hollfinger, Irene; Bartels-Klein, Eireen; Alt, Silvanus & Gerhardt, Holger
