Dynamics of ligands and forces in T cell activation (B06)

• Subject Area: Preparatory and Physical Chemistry of Polymers
• Term: since 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 200049484

Mechanosensing influences ligand recognition, activation and killing mechanisms of T cells. In this project we develop synthetic tools to explore the interplay between mechanical and biochemical interactions at the Antigen Presenting Cell (APC) /T cell interface, and to mimic the dynamics of forces and individual receptor complexes operating at the immunological synapse. Using synthetic hydrogels and micropatterning techniques we engineer minimalistic models of the APC surface with defined ligand types, spatial organization and mechanical engagement. As a unique feature, we use light-driven molecular motors to modulate cytoskeletal/membrane engagement dynamically and in situ, with the possibility to measure the applied forces at molecular level. We study early markers of T cell activation in response to the combinations of ligands and mechanical force.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1027: Physical Modelling of Non-Equilibrium Processes in Biological Systems
• Applicant Institution: Universität des Saarlandes
• Project Head: Professorin Dr. Aránzazu del Campo Bécares