Nutritional reprogramming of chromatin interactions by the glucocorticoid receptor (A21)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2017
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

The Glucocorticoid Receptor (GR) is a ligand-gated transcription factor that controls essential physiological processes at the chromatin level. We identified ANP32A, a high fat diet induced member of the Inhibitor of Histone Acetylation complex (INHAT), as a putative GR coregulator in mouse livers. Our goal is to characterize ANP32A's functions in genomic responses to nutrition and GR signaling. By ChIP- & RNA-seq, we will profile its genome-wide occupancy, linked histone modifications and transcriptional targets. Its impact on chromatin dynamics will be determined by studying both physiological and molecular consequences of hepatic gain and loss of function mouse models, using viral vectors and nanoparticle-coupled siRNAs in vivo.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professorin Dr. Nina Henriette Uhlenhaut