Identification and characterization of thiol switches controlling the yeast metabolic cycle

• Applicant: Professor Dr. Bruce Morgan
• Subject Area: Biochemistry
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 386433891

[1] Elucidate the spatio-temporal dynamics of H2O2 changes during the yeast metabolic cycle.[2] Investigate changes in the thiol peroxidase interactome during the YMC.(i) Use redox proteomics to look for changes in protein thiol oxidation during the YMC(ii) Assess the importance of thiol peroxidases for (changes in) protein thiol oxidation(iii) Employ affinity purification and proximity-associated biotinylation approaches to directly identify thiol peroxidase interaction partners and determine whether the repertoire of interaction partners changes at different stages of the YMC. [3] Biochemically characterize promising candidate thiol switches to determine mechanistically how they affect the YMC

• DFG Programme: Priority Programmes
• Subproject of: SPP 1710: Dynamics of Thiol-Based Redox Switches in Cellular Physiology