Protein–protein interactions at the synapse monitored by quantitative protein cross-linking (A10)

• Subject Area: Biochemistry
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 317475864

State-of-the-art mass spectrometry (MS) makes possible the identification and quantification of cellular proteins, including the detailed analysis of post-translational modifications. MS has recently been combined with chemical cross-linking of proteins, with the purpose of identifying protein–protein contact sites. I now plan to extend cross-linking mass spectrometry to the investigation of protein interactions from native material. I will investigate isolated nerve terminals (synaptosomes) in resting and stimulated (K+/Ca2+ stimulation) states.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1286: Quantitative Synaptology
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Henning Urlaub