Mapping the protein and lipid organization in the plasma membrane of neurons using rapid rupture event imaging (B03)

• Subject Area: Biophysics
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 317475864

Here we want to systematically investigate the impact of protein and lipid composition, curvature and lateral membrane tension on domain formation in multicomponent membranes derived from hippocampal neurons and from synaptosomes. We will map the position and aggregation state of certain proteins and lipids found in the presynaptic membrane with a non-invasive and label-free rapid rupture event imaging, force distance-based atomic force microscopy (AFM).

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1286: Quantitative Synaptology
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Andreas Janshoff