Local signaling for tight coupling of synaptic exo- and endocytosis (A02)

• Subject Area: Cell Biology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 386797833

Maintaining synaptic transmission requires resorting and retrieval of the fused vesicle components by compensatory endocytosis. To address this, we developed "Xenapses", synapses formed by cultured mouse hippocampal neurons on micropatterned host substrates functionalized with synaptogenic proteins. We will now visualize single endocytic events to unravel the mode and mechanism of retrieval, and we will establish protocols for tailored FIB milling and cryo-electron microscopy of purely presynaptic structures. Our work program aims to settle long-standing controversies regarding the endocytic mechanism during synaptic transmission.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1348: Dynamic cellular interfaces: formation and function
• Applicant Institution: Universität Münster
• Project Head: Professor Dr. Jürgen Klingauf