Local signaling for tight coupling of synaptic exo- and endocytosis (A02)

• Subject Area: Cell Biology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 386797833

At the synaptic cellular interface compensatory endocytosis after synaptic vesicle exocytosis is crucial to maintain a pool of release-ready vesicles at predefined release sites. We use our xenapse culture system in conjunction with total internal reflection fluorescence microscopy (TIRFM) and CRISPR-Cas9 genome editing to visualize individual exocytosis and endocytosis events and study the function of release sites and endocytic membrane domains. Cryo-electron tomography of xenapses grown on TEM grids is utilized to correlate function with structure.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1348: Dynamic cellular interfaces: formation and function
• Applicant Institution: Universität Münster
• Project Head: Professor Dr. Jürgen Klingauf