Lipid-mediated activation of protein kinases during cell polarization (A05)

• Subject Area: Cell Biology
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 386797833

We study the dynamic interaction of the basolateral polarity regulators LKB1 and Par-1 with phospholipids of the plasma membrane using mammalian cell culture models and Drosophila in vivo experiments. Furthermore, we apply cryo-electron microscopy and molecular simulations to resolve the molecular mechanism of LKB1/Par-1-phospholipid interaction and its impact on protein localization and kinase activation to understand the establishment of apical-basal polarity.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1348: Dynamic cellular interfaces: formation and function
• Applicant Institution: Universität Münster
• Project Head: Professor Michael Krahn, Ph.D.