Control of herpes simplex virus type 1 specific replication and transmission mechanisms in human dendritic cells
Final Report Abstract
In summary, in our view project was a very successful leading to new and unexpected data, including the fact that autophagic degradation of lamins is essential for the nuclear egress of HSV-1 and that mDC hamper this process, by upregulating KIF1B and KIF2A molecules, and thereby inhibiting the generation of mature infectious viral particles. In addition, we found that HSV-1 derived L-particles modulate the phenotype and stimulatory function of mDCS. Furthermore, targeting IL-6 R expression by HSV-1 on mDCs has been identified as a new viral immune escape mechanism. Finally, we provide data showing that HSV-2 interferes with DC migration and therefore the induction of potent antiviral immune responses. Work regarding mechanisms related to CD83 degradation and the functional ligand studies are still ongoing.
Publications
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(2019). Autophagic degradation of lamins facilitates the nuclear egress of Herpes Simplex Virus type-1. Journal Cell Biology, 218(2):508-523
Turan, Aykut; Grosche, Linda; Krawczyk, Adalbert; Mühl-Zürbes, Petra; Drassner, Christina; Düthorn, Alexandra; Kummer, Mirko; Hasenberg, Mike; Voortmann, Sylvia; Jastrow, Holger; Dörrie, Jan; Schaft, Niels; Kraner, Max; Döhner, Katinka; Sodeik, Beate; Steinkasserer, Alexander & Heilingloh, Christiane Silke
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(2020). Herpes simplex virus type-1 propagation and single-step growth curves. Bio-Protocol 9(23):e3441
Grosche, Linda; Döhner, Katinka; Düthorn, Alexandra; Hickford-Martinez, Ana; Steinkasserer, Alexander & Sodeik, Beate
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(2020). Herpes simplex virus type-2 paralyzes dendritic cell function. Viruses 2020,12, 112
Grosche, Linda; Mühl-Zürbes, Petra; Ciblis, Barbara; Krawczyk, Adalbert; Kuhnt, Christine; Kamm, Lisa; Steinkasserer, Alexander & Heilingloh, Christiane Silke
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(2020). HSV-1 modulates IL-6 receptor expression by human dendritic cells. Front Immunol.; 11:721
Birzer, Alexandra; Krawczyk, Adalbert; Draßner, Christina; Kuhnt, Christine; Mühl-Zürbes, Petra; Heilingloh, Christiane Silke; Steinkasserer, Alexander & Popella, Linda
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(2020). Mass spectrometric characterization of HSV-1 L-particles from human dendritic cells and BHK21 cells and analyses of their functional role. Front. Microbiol. 2020 Sep 29; 11:1997
Birzer, Alexandra; Kraner, Max Edmund; Heilingloh, Christiane Silke; Mühl-Zürbes, Petra; Hofmann, Jörg; Steinkasserer, Alexander & Popella, Linda
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(2020). The CD83 molecule – an important immune checkpoint. Front Immunol.
Grosche, Linda; Knippertz, Ilka; König, Christina; Royzman, Dmytro; Wild, Andreas B.; Zinser, Elisabeth; Sticht, Heinrich; Muller, Yves A.; Steinkasserer, Alexander & Lechmann, Matthias
