Characterization of Cell-Subpopulations in the preeclamptic placenta and decidua
Reproductive Medicine, Urology
Final Report Abstract
Preeclampsia (PE) is a pregnancy disorder manifested by a sudden increase in blood pressure, accompanied by a Proteinuria. PE is the leading cause of maternal and fetal morbidity and mortality and a risk factor for developing cardiovascular diseases in later life. Overall, 5-10% of all pregnancies worldwide develop PE. The placenta is a unique temporary organ, which acts as lungs, liver, gut, kidneys and endocrine glands for the fetus, supplying it with nutrients and oxygen, and harmonizes the cross-talk between the fetal and maternal immune system. The structure of the maternal-fetal interface features a complex relationship between fetal cells and maternal tissue and dysfunctions causes severe consequence for mother and child. It is essential to understand placenta biology on a cellular level, which is now possible with the development of new promising single-cell sequencing technology. Single cell sequencing examines the sequence information from individual cells with optimized next generation sequencing (NGS) technologies. The development of this technology led to the construction of the Human Cell Atlas (HCA) initiative, which aims to create whole-organism tissue maps at the single-cell level. With the previous grant we were funded for the “Characterization of Trophoblast-Subpopulations in first trimester placenta”. In the present project, we were able to analyze first trimester villi and decidua and presented a cell map of the uteroplacental unit in the first trimester of pregnancy. Within this grant, we applied for funding for the “Characterization of Cell-Subpopulations in the preeclamptic placenta and decidua” by single cell sequencing. We investigated cells from control and preeclamptic placenta and decidua and combined the results with the data from the first trimester samples. This approach gave insights in the developing process of the placenta and in mechanisms leading to preeclampsia.
Publications
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Maternal Angiotensin Increases Placental Leptin in Early Gestation via an Alternative Renin-Angiotensin System Pathway. Hypertension, 77(5), 1723-1736.
Nonn, Olivia; Fischer, Cornelius; Geisberger, Sabrina; El-Heliebi, Amin; Kroneis, Thomas; Forstner, Désirée; Desoye, Gernot; Staff, Anne Cathrine; Sugulle, Meryam; Dechend, Ralf; Pecks, Ulrich; Kollmann, Martina; Stern, Christina; Cartwright, Judith E.; Whitley, Guy S.; Thilaganathan, Basky; Wadsack, Christian; Huppertz, Berthold; Herse, Florian & Gauster, Martin
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Disturbed trophoblast transition links preeclampsia progression from placenta to the maternal syndrome. Cold Spring Harbor Laboratory.
Nonn, Olivia; Debnath, Olivia; Valdes, Daniela S.; Sallinger, Katja; Secener, Ali Kerim; Haider, Sandra; Fischer, Cornelius; Tiesmeyer, Sebastian; Nimo, Jose; Kuenzer, Thomas; Maxian, Theresa; Knöfler, Martin; Karau, Philipp; Bartolomaeus, Hendrik; Kroneis, Thomas; Frolova, Alina; Neuper, Lena; Haase, Nadine; Kräker, Kristin ... & Herse, Florian
