Chemical Reporters to study O-GlcNAcylation in Epigenetics (B01)

• Subject Area: Biological and Biomimetic Chemistry
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 325871075

In the second funding period, we want to further develop the newly produced MCR candidates and improve their selectivity. In addition, we want to use these MCRs on neuronal progenitor cells to quantitatively determine the dynamic changes of the O-GlcNAcylomes at defined time points during differentiation. Similarly, changes in O-GlcNAcylation patterns will be detected in murine stem cells in which critical proteins have been knocked down. We also plan to develop synthetic and chemoenzymatic approaches to UDP-GlcNAc analogs of our MCRs to enable their use in complementary chemoenzymatic labeling methods.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1309: Chemical Biology of Epigenetic Modifications
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professorin Dr. Anja Hoffmann-Röder