Genetic dissection of the mechanisms coordinating immunity and metabolism

• Applicant: Professorin Dr. Mirka Uhlirova
• Subject Area: General Genetics and Functional Genome Biology ; Developmental Biology
• Term: from 2018 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 404101084

• Final Report Year: 2022

Final Report Abstract

In summary, our work highlights the cooperation of Multiplexin and Eater as an integral part of a homing mechanism that specifies and maintains hematopoietic sites in Drosophila. It underscores the importance of the physical interaction between the cell and its niche. On the contrary, our data do not support the notion that the formation of the de-novo hematopoietic site on the fat body surface depends on the tissue metabolic status nor sensory neurons and their activity as shown for sessile hematopoietic pockets. With the mounting interest in mechanistic aspects of hematopoietic progenitor-niche interactions in development, homeostasis, and disease and considering the evolutionary conservation of the identified interaction partners, we believe that our findings are significant, relevant and attractive to a broad research community.

Publications

• Ets21c Governs Tissue Renewal, Stress Tolerance, and Aging in the Drosophila Intestine. Cell Reports, 27(10), 3019-3033.e5.
  Mundorf, Juliane; Donohoe, Colin D.; McClure, Colin D.; Southall, Tony D. & Uhlirova, Mirka
  
• JNK-dependent cell cycle stalling in G2 promotes survival and senescence-like phenotypes in tissue stress. eLife, 8 (2019, 2, 8).
  Cosolo, Andrea; Jaiswal, Janhvi; Csordás, Gábor; Grass, Isabelle; Uhlirova, Mirka & Classen, Anne-Kathrin
  
• A Drosophila model to study retinitis pigmentosa pathology associated with mutations in the core splicing factor Prp8. Disease Models & Mechanisms, 13(6).
  Stanković, Dimitrije; Claudius, Ann-Katrin; Schertel, Thomas; Bresser, Tina & Uhlirova, Mirka
  
• Eater cooperates with Multiplexin to drive the formation of hematopoietic compartments. eLife, 9 (2020, 10, 7).
  Csordás, Gábor; Grawe, Ferdinand & Uhlirova, Mirka
  
• Ecd promotes U5 snRNP maturation and Prp8 stability. Nucleic Acids Research, 49(3), 1688-1707.
  Erkelenz, Steffen; Stanković, Dimitrije; Mundorf, Juliane; Bresser, Tina; Claudius, Ann-Katrin; Boehm, Volker; Gehring, Niels H & Uhlirova, Mirka