Project Details
Projekt
Dynamic architecture of Ca2+ microdomains in T cells: disentangling contribution of adenine nucleotides, Ca2+ release and Ca2+ entry (A02)
Subject Area
Immunology
Term
since 2018
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717
Activation of T cells is closely related to Ca2+ microdomains. Our project aims to unravel the interplay of Ca2+ and AN signaling during early T cell activation. Here, we focus on the formation and role of initial localized Ca2+ and ATP signals, the characterization of ‘pure’ NAADP evoked Ca2+ nanodomains, the quantification of initial ATP microdomains after T cell activation as well as the role of initial Ca2+ and ATP microdomains on downstream functions and the T cell fate. To resolve and analyze associated Ca2+ and ATP micro-/nanodomains, the project develops innovative artificial intelligence-based image processing solutions and high-speed super-resolution live cell imaging.
DFG Programme
Collaborative Research Centres
Applicant Institution
Universität Hamburg
Project Heads
Privatdozent Dr. Bjoern-Philipp Diercks, since 1/2020; Professor Dr. René Werner; Dr. Insa Wolf, until 12/2019
