Das Endocannabinoid-System bei der Kontrolle der Futteraufnahme und Energiebilanz während des Übergangs von der Spätträchtigkeit zur Frühlaktation von Milchkühen
Zusammenfassung der Projektergebnisse
The aims of the study were to investigate (1) the association between body fat mobilization and the hypothalamic endocannabinoid system, as well as (2) the effect of i.p. N-arachidonoylethanolamide (AEA) administration on feed intake, orexigenic signaling, taste preference, and fat metabolism of early lactating dairy cows. For the first aim, 16 Holstein cows with high (H) and low (L) degree of fat mobilization were blood sampled during the periparturient period. Both, AEA and 2-AG plasma concentrations increased from late parturition to early lactation, and H cows had higher plasma AEA and NEFA concentrations than L cows in early lactation. Besides, hypothalamic tissue from 9 late lactating (Late) and 10 early lactating (Early) cows was obtained and subjected to immunohistochemical analysis. The protein abundances of the NAPE-PLD and CBR1 were higher and of FAAH lower in Early compared to Late cows. These results suggest that AEA and the hypothalamic endocannabinoid system are strongly regulated and involved in the control of feed intake in periparturient cows. To pursue the second aim, 8 pairs of half-sib Holstein cows were blood sampled 3 weeks antepartum (ap) until 4 weeks postpartum (pp). In the pp period, 8 cows received intraperitoneal injections of 3 µg/kg body weight AEA and 8 cows 0.9% NaCl (CON). Feed and water preference tests and liver and subcutaneous adipose tissue biopsies were performed ap and pp, followed by gas exchanges measurement, brain and tongue tissue sampling in week 4 pp. Daily energy intake, milk yield, and calculated energy balance as well as plasma glucose, NEFA, BHB, HDL, LDL, leptin concentrations revealed no differences between AEA and CON cows. However, AEA cows had higher plasma leptin and TG concentrations on day 21 pp, and tended to have lower PLTP activity and higher BHB concentrations on d 28 pp. AEA administration increased the preference for umamicompared to control and sweet-tasting water and for sucrose- compared to glutamate-containing or control feed. Tongue taste receptor mRNA expression was not different between groups, but CNR1 and OPRD1 mRNA expression in the left amygdala were lower in AEA than CON cows. In the right amygdala, the mRNA expression of OPRK1 was lower in AEA than CON cows, but hypothalamic CNR1, NPY and AGRP transcript levels were not different between groups. The norepinephrine-stimulated glycerol release from cultivated subcutaneous adipose tissue revealed no differences between groups. In the p.p. period, the percentage of fat area in the liver tended to be higher, but hepatic DGAT1 and DGAT2 mRNA expression were lower in AEA than CON cows. The results show that AEA administration in early lactation did not affect feed intake and energy balance but enhanced whole body fat catabolism, fat area in the liver, and taste preference for umami-tasting water and sweet-tasting feed.
Projektbezogene Publikationen (Auswahl)
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Involvement of Plasma Endocannabinoids and the Hypothalamic Endocannabinoid System in Increasing Feed Intake after Parturition of Dairy Cows. Neuroendocrinology, 110(3-4), 246-257.
Kuhla, Björn; Kaever, Volkhard; Tuchscherer, Armin & Kuhla, Angela
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Effects of endocannabinoids on feed intake, stress response and whole-body energy metabolism in dairy cows. Scientific Reports, 11(1).
van Ackern, Isabel; Wulf, Ramona; Dannenberger, Dirk; Tuchscherer, Armin & Kuhla, Björn
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Investigations into taste preference of dairy cows and its modulation by N-arachidonoylethanolamide. Proc. Soc. Nutr. Physiol. 2022, 31:159.
Schwerdtfeger J. Puppe B.; Krause A.; Kuhla B. & Röttgen V.
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Endocannabinoid administration affects taste preference and the expression of cannabinoid and opioid receptors in the amygdala of early lactating cows. Scientific Reports, 13(1).
Schwerdtfeger, Jessica; Krause, Annika; Kalbe, Claudia; Mazzuoli-Weber, Gemma; Eggert, Anja; Puppe, Birger; Kuhla, Björn & Röttgen, Volker
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Lingual and central taste perception of dairy cows and its modulation by N-arachidonoylethanolamide. Proc. Soc. Nutr. Physiol. 2023, 2023, 41.
Schwerdtfeger J. Kalbe C.; Krause A.; Puppe B.; Kuhla B. & Röttgen V.
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The effect of N-arachidonoylethanolamide administration on energy and fat metabolism of early lactating dairy cows. Scientific Reports, 13(1).
Schwerdtfeger, Jessica; Sauerwein, Helga; Albrecht, Elke; Mazzuoli-Weber, Gemma; von Soosten, Dirk; Dänicke, Sven & Kuhla, Björn
