Trafficking and sorting of proteins to the parasitophorous vacuolar membrane of the malaria parasite P. falciparum: Exp1 as a model.
Cell Biology
Final Report Abstract
This project aimed to identify the signals and mechanisms involved in transport of parasite proteins to the PVM, using our previous data as a basis to guide our experimental approach. Indeed, using an alternative epitope tag as opposed to GFP allowed us to identify the transmembrane domain of such proteins as potentially being essential for correct protein sorting and final localisation. The data however also reveal that the situation appears to be more compex than originally thought, as even a transmembrane domain derived from a human protein (glycophorin A) also appears capable or partially driving transport of chimeric reporters to the PVM. Further research will be required to elucidate the fine biophysical details residing in transmembrane domains that allow them to direct transport to specific subcellular localisation, including the PVM. Although the data as it currently stands is worthy of publication, I believe that a more detailed understanding of the role of the TMD in transport will lead to a more highly significant publication with more impact, thus we wish to collect more data before preparing any manuscript.
Publications
-
Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence. PLOS Pathogens, 17(10), e1009969.
Diehl, Mathias; Roling, Lena; Rohland, Lukas; Weber, Sebastian; Cyrklaff, Marek; Sanchez, Cecilia P.; Beretta, Carlo A.; Simon, Caroline S.; Guizetti, Julien; Hahn, Julia; Schulz, Norma; Mayer, Matthias P. & Przyborski, Jude M.
-
Characterization of Plasmodium falciparum macrophage migration inhibitory factor homologue and its cysteine deficient mutants. Parasitology International, 87, 102513.
Schipper, Susanne; Springer, Eric; Hahn, Julia; Rahlfs, Stefan; Bourilhon, Priscila; Bernhagen, Jürgen; Becker, Katja & Przyborski, Jude M.
-
HSP70 and their co-chaperones in the human malaria parasite P. falciparum and their potential as drug targets. Frontiers in Molecular Biosciences, 9.
Barth, Julian; Schach, Tim & Przyborski, Jude M.
-
Investigation of an Allosteric Deoxyhypusine Synthase Inhibitor in P. falciparum. Molecules, 27(8), 2463.
Aroonsri, Aiyada; Wongsombat, Chayaphat; Shaw, Philip; Franke, Siegrid; Przyborski, Jude & Kaiser, Annette
-
Structure and Function of Redox-Sensitive Superfolder Green Fluorescent Protein Variant. Antioxidants & Redox Signaling, 37(1-3), 1-18.
Heimsch, Kim C.; Gertzen, Christoph G.W.; Schuh, Anna Katharina; Nietzel, Thomas; Rahlfs, Stefan; Przyborski, Jude M.; Gohlke, Holger; Schwarzländer, Markus; Becker, Katja & Fritz-Wolf, Karin
-
TLR8 is activated by 5ʹ-methylthioinosine, a Plasmodium falciparum-derived intermediate of the purine salvage pathway. Cell Reports, 39(2), 110691.
Köllisch, Gabriele; Solis, Francisco Venegas; Obermann, Hannah-Lena; Eckert, Jeannine; Müller, Thomas; Vierbuchen, Tim; Rickmeyer, Thomas; Muche, Simon; Przyborski, Jude M.; Heine, Holger; Kaufmann, Andreas; Baumeister, Stefan; Lingelbach, Klaus & Bauer, Stefan
-
Changes in K+ Concentration as a Signaling Mechanism in the Apicomplexa Parasites Plasmodium and Toxoplasma. International Journal of Molecular Sciences, 24(8), 7276.
Dos Santos, Benedito M.; Przyborski, Jude M. & Garcia, Célia R. S.
-
Structure of Leishmania donovani 6-Phosphogluconate Dehydrogenase and Inhibition by Phosphine Gold(I) Complexes: A Potential Approach to Leishmaniasis Treatment. International Journal of Molecular Sciences, 24(10), 8615.
Berneburg, Isabell; Stumpf, Michaela; Velten, Ann-Sophie; Rahlfs, Stefan; Przyborski, Jude; Becker, Katja & Fritz-Wolf, Karin
-
Real-time measurements of ATP dynamics via ATeams in Plasmodium falciparum reveal drug-class-specific response patterns. Antimicrobial Agents and Chemotherapy, 68(5).
Springer, Eric; Heimsch, Kim C.; Rahlfs, Stefan; Becker, Katja & Przyborski, Jude M.
