Metabolic signaling in glioblastoma: a spatial multi-omics approach (C04)

• Subject Area: Hematology, Oncology
• Term: since 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 404521405

Functional cross-talk between metabolism, transcription factor activity and kinase signaling, which critically impacts GB progression by modulating tumor cell malignancy and anti-tumor immunity, and its pharmacological modulation will be investigated by spatial multi-omics in patient-derived organoids and vital patient samples. This project aims at integrating spatial maps obtained by spatial transcriptomics, targeted proteomic and metabolomic mass spectrometry imaging (MSI) as well as MSI-guided laser microdissection and nanoLC-MS for identification, visualization and joint interpretation of spatial molecular profiles associated with response and resistance in glioblastoma.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1389: Understanding and Targeting Resistance in Glioblastoma (UNITE Glioblastoma)
• Major Instrumentation: Microgrid 5 μm upgrade for MSI
• Instrumentation Group: 1790 Spektrometer (Massen-, NMR-, außer 170-178)
• Applicant Institution: Ruprecht-Karls-Universität Heidelberg
• Project Heads: Professor Dr. Carsten Hopf; Dr. Christiane Agnes Opitz