The molecular regulation of chondrogenesis and cartilage homeostasis by MIA/CD-RAP
Orthopaedics, Traumatology, Reconstructive Surgery
Final Report Abstract
Both, in vitro in cell culture systems as well as in vivo in embryonic and adult skeletal system, the expression of the protein MIA/CD-RAP ("melanoma inhibitory activity" or "cartilage-derived retinoic acid sensitive protein") correlates very closely with the differentiation of chondrocytes. The induction of the expression of MIA is affected at the level of transcriptional regulatory mechanisms. As part of the proposed project, additional insights into the function of the protein MIA in the cartilage were gained by in vitro and in vivo experiments. In a previous funding by the DFG the role of MIA in the chondrocyte differentiation was shown, further new target genes such YBX1 and p54nrb were first described. Further analysis could now characterize the molecular function of MIA and MIA target genes in cartilage differentiation exactly. In addition, functional effects of MIA or MIA multimers on differentiation of chondrocytes and progenitor cells and on mechanical properties of cartilage were analyzed.
Publications
-
MIA/CD-RAP Regulates MMP13 and Is a Potential New Disease-Modifying Target for Osteoarthritis Therapy. Cells, 12(2), 229.
Staebler, Sebastian; Lichtblau, Adrian; Gurbiel, Slavyana; Schubert, Thomas; Riechers, Alexander; Rottensteiner-Brandl, Ulrike & Bosserhoff, Anja
