Microscale devices for in situ magnetic resonance enhanced by parahydrogen induced hyperpolarization - µDIMREPHiP
Final Report Abstract
The ability to monitor a chemical process with molecular specificity is appealing across many disciplines where details of transport, chemical conversion, and reaction kinetics are desired. Revealing these details in biological systems is important in the context of determining the development of dysfunctional phenotypes and their response to external stress, while in technical systems knowing these details enables better understanding of reaction progress and homogeneity within chemical reactors. The challenge is in choosing an appropriate analytical technique satisfying the following requirements: non-invasive, non-destructive, broad chemical and physical coverage, high-sensitivity and selectivity. Magnetic resonance (MR) is a technique that addresses three of the four requirements. Sensitivity and selectivity are the primary challenges facing MR; however, by using signal enhancement strategies this can be overcome. The uDIMREPHIP team aimed to develop MR systems capable of revealing the details of chemical processes by simultaneously satisfying the four desired analytical features, choosing the para-hydrogen based MR signal enhancement strategy to tackle the sensitivity challenge. The project had three research objectives (RO): RO-1 was to create a miniaturized gas-liquid contactor with integrated NMR functionality; RO-2 was hyperpolarized substrate delivery to non-trivial samples with minimal transport time; and RO-3 was demonstration of the information gained by hyperpolarized NMR in non-trivial samples. The research objectives were achieved, with novel gas-liquid contactors developed featuring high experimental reproducibility, para-hydrogen hyperpolarization experiments under automation to enhance sample transport time, and bio-compatible para-hydrogen measurement conditions identified for the case of E. coli. The uDIMREPHIP team are confident that the results of this project form a strong basis for future work further pushing the limits of small molecule analytics in the case of challenging measurement conditions.
Publications
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Lenz Lenses in a Cryoprobe: Boosting NMR Sensitivity Toward Environmental Monitoring of Mass-Limited Samples. Analytical Chemistry, 95(2), 1327-1334.
Bastawrous, Monica; Ghosh, Biswas Rajshree; Soong, Ronald; Jouda, Mazin; MacKinnon, Neil; Mager, Dario; Korvink, Jan G. & Simpson, Andre J.
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Flash talk and poster: The 19th European Magnetic Resonance Congress (EU- ROMAR 2023) - Continuous flow PHIP-RASER allows for study of nonlinear phenomena
J. Yang
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Integrating Micro Process Chemistry into an NMR Spectrometer. Chemie Ingenieur Technik, 96(3), 257-278.
Schulte-Hermann, Julia; Yang, Jing; Hurtado, Rivera Andrea C.; Korvink, Jan G.; MacKinnon, Neil & Brandner, Jürgen J.
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Poster: EUROMAR 2023, “NMR compatible bioreactor without background signal”
J. Schulte-Hermann
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Poster: Experimental NMR Conference (ENC 2023), “NMR compatible bioreactor without background signal”;
J. Schulte-Hermann
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Poster: Quantitative NMR Methods for Reaction and Process Monitoring (NMRPM 2023) – The Steady-State PHIP RASER: Generating a Continuous NMR Signal;
J. Yang
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The Steady‐State ALTADENA RASER Generates Continuous NMR Signals**. ChemPhysChem, 24(14).
Yang, Jing; Wang, Peng; Korvink, Jan G.; J., Brandner Jürgen & Lehmkuhl, Sören
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Biomineralization of Electrospun Bacteria-Encapsulated Fibers: A Relevant Step toward Living Ceramic Fibers. ACS Applied Bio Materials, 7(12), 7936-7943.
Schulte-Hermann, Julia; Rießland, Hagen; MacKinnon, Neil; Korvink, Jan G. & Islam, Monsur
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Development of a fully automated workstation for conducting routine SABRE hyperpolarization. Scientific Reports, 14(1).
Yang, Jing; Xin, Ruodong; Lehmkuhl, Sören; Korvink, Jan G. & Brandner, Jürgen J.
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Development of a fully automated workstation for conducting routine SABRE hyperpolarization. Springer Science and Business Media LLC.
Yang, Jing; Xin, Ruodong; Lehmkuhl, Sören; Korvink, Jan G. & Brandner, Jürgen J.
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Enhanced Predictability of Urea Crystallization by an Optimized Laser Repetition Rate. Crystal Growth & Design, 24(9), 3589-3594.
Geiger, Leon; Howard, Ian; MacKinnon, Neil; Forbes, Andrew & Korvink, Jan G.
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Flash talk and poster: The Kyoto Winter School (2024), “Towards Holistic Understanding of Life” - Automatically Hyperpolarizing [1-13C] Pyruvate and Other Substrates through SABRE on Benchtop NMR;
J. Yang
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Magnetic resonance velocimetry of thin falling films. Chemical Engineering Journal, 498, 155260.
Saliba, Georges C.; Korvink, Jan G. & Brandner, Juergen J.
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Magnetic resonance velocimetry reveals secondary flow in falling films at the microscale. Physics of Fluids, 36(7).
Saliba, Georges C.; Korvink, Jan G. & Brandner, Juergen J.
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Talk: ENC 2024, „Advancing towards monitoring bacteria metabolism through SABRE”
J. Schulte-Hermann
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Talk: The 16th International Conference on Gas–Liquid and Gas–Liquid–Solid Reactor Engineering (GLS-16, 2024) – A flow system based on gas-liquid reactor for precise detection of the NMR parameters with NMR hyperpolarization.
J. Yang
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Carbon nanofiber orientation influences bacterial adhesion under flow conditions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 705, 135542.
Schulte-Hermann, Julia; Rießland, Hagen; Hengsbach, Stefan; Korvink, Jan G.; MacKinnon, Neil & Islam, Monsur
