Analyzing the Complexins of the photoreceptor ribbon synapses in mouse retina
Final Report Abstract
In our study, we set out to examine the function of the Complexins (Cplxs) 3 and 4 in photoreceptor ribbon synapses of mouse retina. The key findings of our study are: Cplx3 expression is not regulated by light but by the circadian rhythm. - Cplx4 expression changes light-dependently at the protein but not the transcript level. - Soluble and membrane-associated Cplx3 and Cplx4 pools exist simultaneously in the mouse retina. Their subcellular distribution – soluble versus membrane-associated – does not change light-dependently. - Cplx3 and Cplx4 most likely interact with SVs in a farnesylation-dependent manner. - The Cplx4-SNARE complex interacts with the G-protein Transducin in vitro. - Hypothesis: Transducin, which is known to translocate light-dependently from the outer segment to the synaptic terminal of rod photoreceptors, signals the state of rod photoreceptor adaptation to their ribbon synapse via a putative interaction with the Cplx4- SNARE complex. This interaction throttles the light-dependent replenishment of readily releasable SVs and sustained SV exocytosis.
Publications
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Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina. International Journal of Molecular Sciences, 22(15), 8131.
Lux, Uwe Thorsten; Ehrenberg, Johanna; Joachimsthaler, Anneka; Atorf, Jenny; Pircher, Bianca; Reim, Kerstin; Kremers, Jan; Gießl, Andreas & Brandstätter, Johann Helmut
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Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses. Investigative Opthalmology & Visual Science, 63(3), 21.
Davison, Adam; Gierke, Kaspar; Brandstätter, Johann Helmut & Babai, Norbert
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T-Type Ca2+ Channels Boost Neurotransmission in Mammalian Cone Photoreceptors. The Journal of Neuroscience, 42(33), 6325-6343.
Davison, Adam; Lux, Uwe Thorsten; Brandstätter, Johann Helmut & Babai, Norbert
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A novel calcium channel Cavβ2 splice variant with unique properties predominates in the retina. Journal of Biological Chemistry, 299(4), 102972.
Seitter, Hartwig; Obkircher, Jana; Grabher, Patricia; Hartl, Julia; Zanetti, Lucia; Lux, Uwe Thorsten; Fotakis, Georgios; Fernández-Quintero, Monica L.; Kaserer, Teresa & Koschak, Alexandra
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Complexins: Ubiquitously Expressed Presynaptic Regulators of SNARE-Mediated Synaptic Vesicle Fusion. Advances in Neurobiology, 255-285. Springer International Publishing.
López-Murcia, Francisco José; Reim, Kerstin & Taschenberger, Holger
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Light Adaptation of Rod Photoreceptor Synapses by a Transducin-Complexin-SNARE Complex Interaction. SSRN Electronic Journal.
Lux, Uwe Thorsten; Meyer, Jutta; Jahn, Olaf; Davison, Adam; Babai, Norbert; Gießl, Andreas; Wartenberg, Anna; Sticht, Heinrich; Brose, Nils; Reim, Kerstin & Brandstätter, Johann Helmut
