Laser Scanning Microscope (LSM)

• Subject Area: Medicine
• Term: Funded in 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 442831731

Laser scanning microscopy represents a core expertise of third-party funded and peer-reviewed research projects of the Department of Life Sciences and Chemistry at Jacobs University Bremen and is used since 2002 (HBFG-066-003). The instrument is also instrumental for undergraduate and graduate education of the research and teaching focus area „HEALTH - focus on bioactive substances“. The current laser scanning microscope (LSM) is no longer served and out of date. Therefore, a new LSM is required, which will be used by up to 50 different users of the applying research groups and integrated junior groups. The LSM is essential to answer a range of important research questions of the interdisciplinary applicants. Thus, the application spectrum ranges from high-resolution fluorescence microscopy to automated screening-options (image acquisition in the tile-imaging mode) to imaging of living cells under well-controlled temperature and CO2 conditions. Therefore, an LSM system is foreseen that is based on an inverted and motorized research microscope, equipped with epifluorescence and bright field imaging. High-end multi-channel image acquisition in photon-counting and integrative modes as well as fluorescence correlation spectroscopy is required. Due to the multi-user requirements, the software shall allow for multiparameter experiments, and the image analysis modules need to comprise colocalisation and 3D-reconstructions.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Laser Scanning Mikroskop (LSM)
• Instrumentation Group: 5090 Spezialmikroskope
• Applicant Institution: Constructor University Bremen
• Leader: Professorin Dr. Klaudia Brix