Poxvirus transcription: Structural and functional characterization of Vaccinia virus initiation and initially transcribing complexes

• Applicant: Professor Dr. Utz Fischer
• Subject Area: Biochemistry
• Term: from 2020 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 445237915

• Final Report Year: 2025

No abstract available

Publications

• Structural Basis of Poxvirus Transcription: Transcribing and Capping Vaccinia Complexes. Cell, 179(7), 1525-1536.e12.
  Hillen, Hauke S.; Bartuli, Julia; Grimm, Clemens; Dienemann, Christian; Bedenk, Kristina; Szalay, Aladar A.; Fischer, Utz & Cramer, Patrick
  
• Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes. Cell, 179(7), 1537-1550.e19.
  Grimm, Clemens; Hillen, Hauke S.; Bedenk, Kristina; Bartuli, Julia; Neyer, Simon; Zhang, Qian; Hüttenhofer, Alexander; Erlacher, Matthias; Dienemann, Christian; Schlosser, Andreas; Urlaub, Henning; Böttcher, Bettina; Szalay, Aladar A.; Cramer, Patrick & Fischer, Utz
  
• Structural basis of the complete poxvirus transcription initiation process. Nature Structural & Molecular Biology, 28(10), 779-788.
  Grimm, Clemens; Bartuli, Julia; Boettcher, Bettina; Szalay, Aladar A. & Fischer, Utz
  
• Structure and function of the poxvirus transcription machinery. The Enzymes, 1-20. Elsevier.
  Fischer U., Bartuli J. & Grimm C.
  
• A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells. STAR Protocols, 3(1), 101116.
  Bartuli, Julia; Lorenzi, Isotta; Backes, Simone; Grimm, Clemens & Fischer, Utz
  
• Cytoplasmic gene expression: lessons from poxviruses. Trends in Biochemical Sciences, 47(10), 892-902.
  Grimm, Clemens; Bartuli, Julia & Fischer, Utz
  
• The Vaccinia Virus DNA Helicase Structure from Combined Single-Particle Cryo-Electron Microscopy and AlphaFold2 Prediction. Viruses, 14(10), 2206.
  Hutin, Stephanie; Ling, Wai Li; Tarbouriech, Nicolas; Schoehn, Guy; Grimm, Clemens; Fischer, Utz & Burmeister, Wim P.
  
• Structure and flexibility of the DNA polymerase holoenzyme of vaccinia virus. PLOS Pathogens, 20(5), e1011652.
  Burmeister, Wim P.; Boutin, Laetitia; Balestra, Aurelia C.; Gröger, Henri; Ballandras-Colas, Allison; Hutin, Stephanie; Kraft, Christian; Grimm, Clemens; Böttcher, Bettina; Fischer, Utz; Tarbouriech, Nicolas & Iseni, Frédéric