NISED - A Nanoparticle Immunoassay based on Single-entity Electrochemical Detection
Final Report Abstract
In the context of the project “NISED – A Nanoparticle Immunoassay based on Single-entity Electrochemical Detection,” a novel electrochemical sensing platform based on the detection of individual nanoparticle impacts was developed and investigated. The focus was on the investigation of a competitive binding assay using functionalized silver nanoparticles in a lateral flow format. In this approach, target-functionalized nanoparticles served as redox-active markers, and the excess nanoparticles were detected electrochemically after competition with a free target on coated beads. Furthermore, the project realized a second biosensor concept by integrating an enzyme-based biosensing module. Utilizing the collateral cleavage activity of Cas12a, DNA-modified silver nanoparticles could be selectively released from magnetic bead complexes and detected by stochastic impact electrochemistry. This strategy enables digital, amplification-free detection of nucleic acid targets with high specificity and low detection limits. The project also explored in depth the effects of nanoparticle surface chemistry on redox activity and signal transduction. Specifically, the influence of ligand structure and terminal functional groups on electron transfer kinetics and detection efficiency was analyzed. In parallel, innovative microelectrode arrays were developed using scalable fabrication methods, including printing and laser ablation. These structures enabled sensitive and spatially resolved nanoparticle impact measurements in microfluidic systems. The technologies developed in the project include new electrode-based microsensor systems, advanced nanoparticle functionalization, and nanoparticle detection schemes. The project successfully combines sensor system development, and single-impact electrochemistry and contributes to the advancement of digital biosensing technologies for future point-of-care diagnostics.
Link to the final report
https://mediatum.ub.tum.de/1832043
Publications
-
Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay. ACS Sensors, 7(7), 1967-1976.
Weiß, Lennart J. K.; Rinklin, Philipp; Thakur, Bhawana; Music, Emir; Url, Heike; Kopic, Inola; Hoven, Darius; Banzet, Marko; von Trotha, Tassilo; Mayer, Dirk & Wolfrum, Bernhard
-
Inkjet-printed 3D micro-ring-electrode arrays for amperometric nanoparticle detection. Nanoscale, 15(8), 4006-4013.
Peng, Hu; Grob, Leroy; Weiß, Lennart Jakob Konstantin; Hiendlmeier, Lukas; Music, Emir; Kopic, Inola; F. Teshima, Tetsuhiko; Rinklin, Philipp & Wolfrum, Bernhard
-
Low‐Cost, On‐Site, Nano‐Impact Detection of Silver Nanoparticles via Laser‐Ablated Screen‐Printed Microelectrodes. Advanced Materials Technologies, 8(10).
Grob, Leroy; Weiß, Lennart J. K.; Music, Emir; Schwertfeger, Ilja; Al Boustani, George; Feuerbach, Julian; Nikić, Marta; Hiendlmeier, Lukas; Rinklin, Philipp & Wolfrum, Bernhard
-
Stochastic Detection of Silver Nanoparticles for Sensing Applications
L. J. K. Weiß
-
Digital CRISPR-Powered Biosensor Concept without Target Amplification Using Single-Impact Electrochemistry. ACS Sensors, 9(11), 6197-6206.
Freko, Sebastian; Nikić, Marta; Mayer, Dirk; Weiß, Lennart J. K.; Simmel, Friedrich C. & Wolfrum, Bernhard
-
Exploring DNA Functionalization Techniques for Silver Nanoparticles. Advanced Materials Interfaces, 12(12).
Freko, Sebastian; Nikić, Marta; Weiß, Lennart J.K. & Wolfrum, Bernhard
-
Stochastic Impact Electrochemistry of Alkanethiolate‐Functionalized Silver Nanoparticles. Small, 21(16).
Weiß, Lennart J. K.; Nikić, Marta; Simmel, Friedrich C. & Wolfrum, Bernhard
