Project Details
Projekt
Organic matter: Abiotic transformations and microbial interactions
Subject Area
Oceanography
Term
since 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 431491505
DynaDeep will unravel the functionality and relevance of marine-terrestrial interactions below high-energy beaches, where we propose the deep subsurface to act as a dynamic bioreactor and unique microbial habitat affecting elemental net fluxes to the sea. To reach this goal, six subprojects will cooperate in joint field campaigns, shared sampling approaches as well as experimental work and use mathematical models in an integrative approach. Subproject P3 will study the sources, composition and transformations of organic matter as the main drivers of biogeochemical processes in the deep subterranean estuary. We hypothesize that the transient character of the deep subsurface is crucial for the turnover and molecular transformation of organic matter through interacting abiotic and biotic processes. A combination of targeted laboratory experiments and field work will be applied in order to identify and characterize (1) potential endmembers of organic matter supplied to the deep subterranean estuary, (2) abiotic changes of dissolved organic matter (DOM) quantity and composition occurring at temporally and spatially variable redox interfaces, and (3) degradation and transformation of DOM by microbial communities. Detailed molecular information will be used to decipher linkages between DOM and microbial community composition and activity. Organic matter composition will be characterized by state-of-the-art molecular approaches such as ultrahigh resolution Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and ultra-performance liquid chromatography, and complemented by analyses of stable and radiocarbon isotopes. Molecular proxies will be established as diagnostic tools for specific biogeochemical states of the deep subsurface and the resulting microbial niche space. Results from P3 will populate the coupled reactive transport hydrogeological model (P6) with reactivity terms and provide information on sources and age of DOM (P1). Molecular data acquired in P3 will be interpreted in the context of microbial community data (P5) and distribution of key trace elements (P4), and will contribute to a mechanistic understanding of microbial respiration (P2).
DFG Programme
Research Units
International Connection
USA
Cooperation Partner
Professor Dr. Timothy J. Shaw
