The photosynthetic response to iron limitation and iron resupply in diatoms
Zusammenfassung der Projektergebnisse
The research conducted during this project has led to several comprehensive pieces of work, mostly published in peer-reviewed journals. One significant aspect of the work is the extensive characterization of the gene family encoding for the light-harvesting proteins from P. tricornutum. The work on gene expression revealed fcp genes that are specifically responsive to iron limitation and others that are more responsive to oxidative stress in general. Most of the work conducted during this grant focussed on the genome T. oceanica. This undertaking was very worthwhile, judged from the level of interest that the genome browser has generated among the researchers working on diatoms. The characterization of the chloroplast genome as well as the nuclear genome provide a new working model for diatom research that is based on a highly ecologically relevant species. T. oceanica, an diatom that prefers oligotrophic oceanic environments is well suited to understanding ecological problems such as survival in low nutrient and low dissolved iron environments. Several aspects of the response to iron limitation are of special interest. We have discovered that many of the genes highly expressed under iron limitation in P. tricornutum have either been duplicated or have repeated elements in T. oceanica. We are further investigating these proteins, mainly contained in the new protein families ISP1, ISIP2, ISIP3. Overeall, the goals of the project were realized. However, some aspect of the proposed work, the metabolomics results for example, turned out to be less informative than originally planned, while the proteomics approach, which was not initally proposed, contributed significantly to the annotation and interpretation of the genome and transcriptome information.
Projektbezogene Publikationen (Auswahl)
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(2008) The Phaeodactylum genome reveals the dynamic nature and multilineage evolutionary history of diatom genomes. Nature 456: 239-24
Bowler C, Allen AE, Badger JH, Grimwood J, Jabbari K, Kuo A, Maheswari U, Martens C, Maumus F, Otillar RP, Rayko E, Salamov A, Vandepoele K, Beszteri B, Gruber A, Heijde M, Katinka M, Mock T, Valentin K, Verret F, Berges JA, Brownlee C, Cadoret J-P, Chiovitti A, Choi CJ, Coesel S, De Martino A, Detter JC, Durkin C, Falciatore A, Fournet J, Haruta M, Huysmann M, Jenkins BD, Jiroutova K, Jorgensen RE, Joubert Y, Kaplan A, Kroeger N, Kroth P, La Roche J, Lindquist E, Lommer M, Martin-Jezequel V, Lopez PJ, Lucas S, Mangogna M, McGinnis K, Medlin LK, Montsant A, Oudot-Le Secq M-P, Napoli C, Obornik M, Petit J-L, Porcel BM, Poulsen N, Robison M, Rychlewski L, Rynearson TA, Schmutz J, Schnitzler Parker M, Shapiro H, Siaut M, Stanley M, Sussman MJ, Taylor A, Vardi A, von Dassow P, Vyverman W, Willis A, Wyrwicz LS, Rokhsar DS, Weissenbach J, Armbrust EV, Green BR, Van de Peer Y, Grigoriev IV
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2008. The global cellular response of a marine diatom to iron limitation. Proc. Nat. Acad. Sci. 105:10438-10443
Allen AE, LaRoche J, Maheswari U, Lommer M, Schauer N, Lopez P, Finazzi G, Fernie AR, Bowler C
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2010. Digital expression profiling of novel diatom transcripts provides insight into their biological functions. Genome Biology 11 Art. No. R85 2010
Maheswari U, Jabbari K, Petit JL, Porcel BM, Allen AE, Cadoret JP, De Martino A, Heijde M, Kaas R, La Roche J, Lopez PJ, Martin-Jezequel V, Meichenin A, Mock T, Parker MS, Vardi A, Armbrust EV, Weissenbach J, Katinka M, Bowler C
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2010. Recent transfer of an iron-regulated gene from the plastid to the nuclear genome in an oceanic diatom adapted to chronic iron limitation. BMC Genomics 11:718
Lommer M, Alexandra-Sophie Roy, Markus Schilhabel, Stefan Schreiber, Philip Rosenstiel, Julie LaRoche
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2012. Genome and low-iron response of an oceanic diatom adapted to chronic iron limitation. Genome Biol. Jul 26;13(7):R66
Lommer M, Specht M, Roy AS, Kraemer L, Andreson R, Gutowska MA, Wolf J, Bergner SV, Schilhabel MB, Klostermeier UC, Beiko RG, Rosenstiel P, Hippler M, LaRoche J
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Acclimation and adaptation to low-iron conditions in the marine diatoms Phaeodactylum tricornutum and Thalassiosira oceanica, PhD thesis 2012, Christian Albrechts University, 318 pp.
Lommer, Markus
