Capillary Sequencer

• Subject Area: Agriculture, Forestry and Veterinary Medicine
• Term: Funded in 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 458332906

High-resolution capillary electrophoresis is a standard method for precise identification of DNA polymorphisms. The main application of the capillary sequencer is the high-throughput genotyping of locus-specific DNA markers such as highly variable nuclear microsatellite markers in different plant and animal species. The instrument will also be used in ongoing and proposed projects for the sequencing of specific DNA-barcoding regions in a variety of different organismal groups. High-precision genotyping is indispensable for population genetic and forensic application such as gene flow and parentage analyses and for the assessment of genetic variation. This method will be used also in the future as standard method for the monitoring of genetic resources and of human impacts on biological diversity in the framework of conservation programs. The analysis of larger sample sizes at representative genetic markers is becoming even more important for the publication in renowned scientific journals. The new instrument would allow the analysis of larger sample sizes in multiplex reactions (several thousand samples per week) as anticipated in ongoing and proposed projects. Because of the wide spectrum of genotyping methods (e.g., microsatellites, SNPs, genome-wide AFLPs) and the sequencing of DNA-barcoding regions, the instrument would be used by different research groups of different faculties (Forestry, Biology), securing a continuous utilization of the system. The instrument would become part of a service unit which also includes a Mass spectrometer for the large-scale assessment of Single Nucleotide Polymorphisms (SNPs) and of epigenetic variation.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: Kapillarsequenzierer
• Instrumentation Group: 3150 DNA-Sequenzer
• Applicant Institution: Georg-August-Universität Göttingen
• Leader: Professor Dr. Oliver Gailing