High-resolution electron microscopy of macro-molecular complexes in cell cycle regulation (A04)

• Subject Area: Biochemistry ; Structural Biology ; Cell Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 424228829

Gatsogiannis will use cryo-electron microscopy for a structure-function analysis of various key protein complexes in cell state transitions. He will (1) determine how substrate-engaged p97 cooperates with accessory factors involved in DNA repair and checkpoint control. He will (2) dissect a novel p97-HTRA1 complex that couples substrate unfolding and proteolysis. And (3), he will obtain structural insights into the molecular machinery that mediates and regulates mitotic spindle attachment to the chromosomal kinetochores to unravel how these factors co-operate to trigger metaphase to anaphase transition during cell division.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1430: Molecular Mechanisms of Cell State Transitions
• Applicant Institution: Universität Duisburg-Essen
• Project Head: Professor Dr. Christos Gatsogiannis