Molecular insights into the role of MTREC in ncRNA processing and degradation (B03)

• Subject Area: Structural Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 439669440

Non-coding RNAs or un-/mis-spliced mRNAs are rapidly recognized by adaptor complexes and targeted to the nuclear exosome. Here, we continue our work on the structure and function of the MTREC (Mtl1-Red1 core) and CNM (Ctr1-Nrl1-Mtl1) adaptors with special focus on their RNA target recognition due to aberrant processing or modification. CNM recognizes stalled spliceosomes and opens a link to quality control. We use an integral structural biology approach including split-tag purification or in vitro reconstitution, crosslinking mass-spec and RNA-seq technologies, and cryo-EM and X-ray analyses.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 319: RMaP: RNA Modification and Processing
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professorin Dr. Irmgard Sinning