Nanometric architecture of the synaptic cytoskeleton (A12)

• Subject Area: Structural Biology ; Molecular Biology and Physiology of Neurons and Glial Cells
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 317475864

During the current funding period we have established a workflow to efficiently image synapses by cryo-electron tomography (cryo-ET). We will capitalize on these developments to investigate the native organization of the synaptic cytoskeleton. We will systematically map the organization of synaptic actin and microtubules and their associations with all major synaptic components. Altogether, we aim to provide a detailed cartography of the synaptic cytoskeleton and its roles in synaptic function.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1286: Quantitative Synaptology
• Applicant Institution: Georg-August-Universität Göttingen
• Project Head: Professor Dr. Ruben Fernandez Busnadiego