Compositional and structural analysis of DNA replication origins by locus-specific chromatin isolation (A29*)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

Chromatin dynamics and the local chromatin organization critically contribute to the activity of DNA replication origins, but the precise chromatin and structural features like the exact nucleosome configuration, histone modification state or requirement of additional chromatin factors to allow efficient and timely replication initiation remain largely elusive. In this project, we will use locus-specific chromation isolation to target four distinct replication origins on yeast chromosome III that show major differences in their intrinsic replication properties regarding timing and firing efficiency. A combination of biochemical and functional assays is proposed to study whether the local chromatin structure determines the capacity of these regions to initiate DNA replication in vivo.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Dr. Stephan Hamperl