Untersuchung der Rolle von Zell-Zell-Interaktionen bei der Entstehung von Herzrhythmusstörungen
Zusammenfassung der Projektergebnisse
The project aimed to investigate the cell-cell interactions between cardiac immune cells, especially macrophages, and cardiomyocytes in order to better understand the mechanisms behind myocardial remodelling, cardiomyocyte transplantation, and cardiac arrhythmogenesis. We planned to investigate the molecular and electrophysiological interactions of cardiac macrophages with adult cardiomyocytes from the healthy and ischemic heart. However, technical challenges prevented us from obtaining a sufficient number of CCR2+ and CCR2- macrophages for co-culture experiments. Hence, we tested our hypothesis that significantly different interactions between the transplanted cardiomyocytes and the cardiac macrophages could lead to varied functional outcomes in the in-vivo setting. We were able to demonstrate that the improved functional outcome following cardiomyocyte transplantation is dependent on a specific CCR2 macrophage response 1. Additionally, we developed an in-vitro system using isolated neonatal murine cardiomyocytes to investigate the effects of different drugs on cell-cell conduction and drug response using microelectrode arrays.
Projektbezogene Publikationen (Auswahl)
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The Effects of Hypoxic Preconditioned Murine Mesenchymal Stem Cells on Post-Infarct Arrhythmias in the Mouse Model. International Journal of Molecular Sciences, 23(16), 8843.
Ahmad, Beschan; Skorska, Anna; Wolfien, Markus; Sadraddin, Haval; Lemcke, Heiko; Vasudevan, Praveen; Wolkenhauer, Olaf; Steinhoff, Gustav; David, Robert & Gaebel, Ralf
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Cardiac cell therapies for the treatment of acute myocardial infarction in mice: systematic review and meta-analysis. Cytotherapy, 25(6), 640-652.
Lang, Cajetan Immanuel; Dahmen, Anika; Vasudevan, Praveen; Lemcke, Heiko; Gäbel, Ralf; Öner, Alper; Ince, Hüseyin; David, Robert & Wolfien, Markus
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CCR2 macrophage response determines the functional outcome following cardiomyocyte transplantation. Genome Medicine, 15(1).
Vasudevan, Praveen; Wolfien, Markus; Lemcke, Heiko; Lang, Cajetan Immanuel; Skorska, Anna; Gaebel, Ralf; Galow, Anne-Marie; Koczan, Dirk; Lindner, Tobias; Bergmann, Wendy; Mueller-Hilke, Brigitte; Vollmar, Brigitte; Krause, Bernd Joachim; Wolkenhauer, Olaf; Steinhoff, Gustav & David, Robert
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New Approaches in Heart Research: Prevention Instead of Cardiomyoplasty?. International Journal of Molecular Sciences, 24(10), 9017.
Gaebel, Ralf; Lang, Cajetan; Vasudevan, Praveen; Lührs, Larissa; de Carvalho, Katherine Athayde Teixeira; Abdelwahid, Eltyeb & David, Robert
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Multi-modal assessment of a cardiac stem cell therapy reveals distinct modulation of regional scar properties. Journal of Translational Medicine, 22(1).
Schweins, Moritz; Gäbel, Ralf; Raitza, Matti; Vasudevan, Praveen; Lemcke, Heiko; Joksch, Markus; Schildt, Anna; Kurth, Jens; Lindner, Tobias; Meinel, Felix G.; Öner, Alper; Ince, Hüseyin; Vollmar, Brigitte; Krause, Bernd Joachim; David, Robert & Lang, Cajetan Immanuel
